Liver proliferating cell nuclear antigen, BAX/Bcl-2 ratio, collagen, and polysaccharide accumulation as diagnostic tools in experimental hepatocellular carcinoma

BAX/Bcl-2 ratio, polysaccharide, collagen, and PCNA in hepatocellular carcinoma diagnosis


  • Nabil Mohie Abdel-Hamid Department of Biochemistry, Faculty of Pharmacy, Kafrelsheikh University, Egypt.
  • Mohamed K Hassan Department of Biotechnology, Faculty of Science, Port Said University, Egypt.
  • Amal AM Ahmed Department of Cytology and Histology, Faculty of Veterinary Medicine, Suez Canal University, Egypt.
  • Sara Gamal Abd Allah Department of Biotechnology, Faculty of Science, Port Said University, Egypt.
  • Nahla H. Anber Fellow of Biochemistry in the Emergency Hospital, Mansoura University, Mansoura, Egypt.



Hepatocellular carcinoma, Caspase-3, Cellular proliferation, Apoptosis, Collagen, Polysaccharides


Objectives:  This experimental study was conducted to look for a sensitive diagnostic panel for early detection of HCC.

Methods: Combination of diethyl nitrosamine (DENA, 200 mg/kg, IP, once), two weeks later, CCl4(3ml/kg/week), subcutaneously, for 6 weeks) induced HCC in rats. Sixteen male Wistar rats were divided into 2 groups, control, and HCC.

Results: DENA plus CCl4 elevated serum alpha-fetoprotein, liver enzyme activity and depressed superoxide dismutase, reduced glutathione (GSH), elevated malondialdehyde. Also, depressed caspase-3, elevated collagen, polysaccharide accumulation, proliferating cell nuclear antigen in liver tissues, and depressed BAX / Bcl-2 ratio.   

Conclusion: Decreased BAX/Bcl-2 ratio, elevated collagen deposition, polysaccharide accumulation, nuclear proliferation, and tissue oxidative stress help in the early diagnosis of liver cancer. 


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How to Cite

Abdel-Hamid , N. M. ., Hassan , M. K. ., Ahmed, A. A. ., Abd Allah, S. G. ., & Anber, N. H. . (2022). Liver proliferating cell nuclear antigen, BAX/Bcl-2 ratio, collagen, and polysaccharide accumulation as diagnostic tools in experimental hepatocellular carcinoma : BAX/Bcl-2 ratio, polysaccharide, collagen, and PCNA in hepatocellular carcinoma diagnosis. Journal of Contemporary Medical Sciences, 8(1).