Comparison of Folliculogenesis and Follicular Maturation in Norvegicus Rats using Foeniculum Vulgare and Clomiphene Citrate

Authors

  • Maha M. Kadhim Al-Tu’ma Department of Anaesthesia Techniques, College of Health and Medical Techniques, Al-Zahraa University for Women, Kerbala, Iraq.
  • Mays A. Dakhel Al-Turihi Department of Anaesthesia Techniques, College of Health and Medical Techniques, Al-Zahraa University for Women, Kerbala, Iraq.
  • Sura Zaki Ghafat Department of Physiotherapy Techniques, College of Health and Medical Techniques, Al- Zahraa University for Women, Kerbala, Iraq.

DOI:

https://doi.org/10.22317/jcms.v8i2.1205

Keywords:

Foeniculum, Rattus norvegicus, Rats, Wistar, follicular maturation, Clomiphene Citrate

Abstract

Objective: The objective of this work is to understand the importance of follicular maturation in the reproductive life of the Wistar's rats (Rattus norvegicus) using Foeniculum Vulgare (Fennel) and Clomiphene Citrate as reference drug.

Methods: Foeniculum Vulgare (Fennel) are used in doses of (400, 800mg/Kg), Clomiphene Citrate along with distilled water administration. The sexual hormones involved in the oestrous cycle, folliculogenesis, the role of the ovaries, the embryonic and fetal development of the ovary are addressed. An extract of Fennel was obtained using ethyl alcohol by miscreation. After successful administration, a histological study was also conducted on 40 rats grouped into four categories. Statistical analysis also applied standard parameters like chi-square, Tukey test and variance test.

Results: It was identified that after administration of Fennel citrate drug along with distilled waters, significant differences (p<0.05) were identified in the rats and all rats formed primary (90% given 800mg/kg after fennel administration), secondary and matured (80% given 800mg/kg after fennel administration) follicles.

Conclusion: The effect of F. Vulgare at dose of 400mg was not influential as compared to F. Vulgare at 800mg. The clomiphene citrate increased the number of primary follicles in all of the rats studied. In contrast, the rats that ate F. Vulgare, had secondary and mature follicles predominating and did not have any primary follicles.

References

- Dehghani F, Panjehshahin MR, Mirzaee Z, Mehrabani D. Effect of Foeniculum vulgare organic extract on blood sex hormones and reproductive tissues of male rats. Journal of Applied Animal Research. 2005 Mar 1;27(1):17-20.

- Gardner Zoë, Michael McGuffin, (2013) American Herbal Products Association's Botanical Safety Handbook, CRC Press; 2nd edition Page. 370, ISBN-13: 978-1466516946

- Kilic-Okman T, Kucuk M, Altaner S. Comparison of the effects of letrozole and clomiphene citrate on ovarian follicles, endometrium, and hormone levels in the rat. Fertility and sterility. 2003 Dec 1;80(6):1330-2.

Published

2022-04-26

How to Cite

Al-Tu’ma, M. M. K. ., Al-Turihi, M. A. D. ., & Ghafat, S. Z. . (2022). Comparison of Folliculogenesis and Follicular Maturation in Norvegicus Rats using Foeniculum Vulgare and Clomiphene Citrate. Journal of Contemporary Medical Sciences, 8(2). https://doi.org/10.22317/jcms.v8i2.1205