Molecular analysis of Carbapenem resistant genes in Pseudomonas aeruginosa isolated from Baghdad hospitals

Authors

  • Ali H. Salih Department of Medical Microbiology, Faculty of Medicine, University of Al-Qadisiyah, Al-Qadisiyah, Iraq.
  • Adnan H. Aubaid Department of Medical Microbiology, Faculty of Medicine, University of Al-Qadisiyah, Al-Qadisiyah, Iraq.
  • Ghada B. Ali Department of Medical Microbiology, Faculty of Medicine, University of Al-Qadisiyah, Al-Qadisiyah, Iraq.

DOI:

https://doi.org/10.22317/jcms.v8i4.1257

Keywords:

Carbapenems, Pseudomonas aeruginosa, Hospitals, Iraq

Abstract

Objectives: This study aimed to molecularl investigation of  prevalence the carbapenem-resistant genes in P. aeruginosa in isolates collected from Baghdad hospitals.

Methods: In a cross-sectional manner, P. aeruginosa were isolated and identified from patients who attended to Hospital in Baghdad city during the period of   December 2021 to June 2022. Genotypic characterization  of 16SrRNA gene, plasmid profile, exoA gene, carbapenem resistance gene were tested.

Results: Diagnosis of P. aeruginosa isolates was confirmed genotypically via the amplification of 16SrRNAgene with 1504 bp by using PCR amplification of genes encoding Ambler class B MBL (blaIMP, blaVIM,IMI,blaNDM, blaSPM-1, and blaGIM), and Ambler class D carbapenemase (blaOXA-23,blaOXA-24, blaOXA-40, blaOXA-48,blaOXA-50, blaOXA-51andblaOXA-58) were performed. The carbapenem resistant isolates were also evaluated for the presence of class D carbapenemase (blaOXA 50), genes by PCR.

Conclusion: This study proved that P. aeruginosa isolated had carbapenem-resistant genes that strongly correlated with antibiotic resistance according to phenotypic and genotypic characterization.

References

Rashid, A., Akram, M., Kayode, O.T. and Kayode, A.A. (2020). Clinical features and epidemiological patterns of infections by multidrug resistance Staphylococcus aureus and Pseudomonas aeruginosa in Patients with Burns. Biomed. J. Sci. Tech. Res. 25(4): 19272- 19278.

Jeong, S .J.;Yoon, S. S.; Bae, I. K.; Jeong, S. H.; Kim, J. M.; Lee, K. (2014). Risk factors For mortality in patients with bloodstream infections caused by cabapenem-Resistant Pseudomonas aeruginosa: clinical impact of bacterial virulence and Strain on outcome. DiagnMicrobiol Infect Dis; 80:130-135.

Vural, E.; Delialioglu, N.; Ulger, S. T.; Emekdas, G. and Serin, M. S. (2020). Phenotypic and molecular detection of the metallo-beta-lactamases in carbapenem-resistant Pseudomonas aeruginosa isolates from clinical samples. Jundishapur, J. Microbiol. e90034: 1-8. 10.5812/jjm.90034.

Rostami, S.; Sheikh, A. F.; Shoja, S.; Farahani, A.; Tabatabaiefar, M. A.; Jolodar, A. and Sheikhi, R. (2018). Investigating of four main carbapenem-resistance mechanisms in high-level carbapenem resistant Pseudomonas aeruginosa isolated from burn patients. J. Chin. Med. Ass., 81: 127-132.

Collee, J.G. Fraser, A.G.; Marmion, B.P. etal. (1996). Mackie and McCartney Practical Medical Microbiology. 14th ed., Churchill Livingstone, New York, pp. 413-423.

Jiang,H. Dong, H. Zhang, G. et al. (2006). Microbial diversity in water and sediment of Lake Chaka, an athalassohaline lake in northwestern China. Appl EnvironmenMicrobiol., 72(6): 3832–3845.

Woodford N., Ellington M.J., Coelho J.M., Turton J.F., Ward M.E., Brown S., Amyes S.G., Livermore D.M. Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. Int. J. Antimicrob. Agents. 2006;27:351–353. doi: 10.1016/j.ijantimicag.2006.01.004. [PubMed] [CrossRef] [Google Scholar].

Sambrook, J. and Russel, D. W. (2001). Molecular cloning: A laboratory manual. (3rded). Cold Spring Harbor, USA. Pp.: 5-52.

Strich JR, Warner S, Lai YL, et al. Needs assessment for novel Gram-negative antibiotics in US hospitals: a retrospective cohort study. Lancet Infect Dis 2020.

Langendonk, R.F., Neill, D.R. and Fothergill, J.L. (2021). The building blocks of antimicrobial resistance in Pseudomonas aeruginosa: Implications for current resistance-breaking therapies. Front. Cell. Infect. Microbiol., 11 (April): 1-22.

Alornaaouti, A. (2015). Study of genotyping and some virulence factors of Pseudomonas aeruginosa. M.Sc. Thesis, Baghdad University, Pure Sciences -Ibn Al-Haitham Collage. P.49-79.

Brooks, G.F.; Carroll, K.C.; Butel, S.J.etal. (2013). Jawetz, Melnick, and Adelbergs, Medical Microbiology. 26th ed. McGraw-Hill. United States.

Botelho, J., Grosso, F. and Peixe, L. (2018). Unravelling the genome of a Pseudomonas aeruginosa isolate belonging to the high-risk clone ST235 reveals an integrative conjugative element housing a blaGES-6 carbapenemase. J. Antimicrob. Chemother., 73: 77-83.

Al-Janahi, H.C. (2020). Occurrence and molecular characterization of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa in Najaf hospitals. M.Sc. Thesis. Faculty of Medicine. University of Kufa. Iraq.

Rasool, A.A., Almohana, A.M., Alsehlawi, Z.S., Abed Ali, I., Al-Faham, M. and Al-Sherees, H.A. (2021). Molecular detection of carbapenems resistance genes in Pseudomonas aeruginosa isolated from different hospitals in Najaf, Iraq.

Alsaady, F. (2021). Dissemination and Molecular Characterization of Extensively Drug-Resistant (XDR) Pseudomonas aeruginosa in Najaf Province. M.Sc. Thesis College of medicine, Al-Kufa University, Iraq. P.70-86. .Aubaid AH.;Mahdi ZH.; Abd-Alraoof NM. And Jabbar NM.(2020).Detection of mec A ,van A andvan B genes of Staphylococcus aureus isolated from patients in Al-Muthanna province Hospitals.Indian Journal of Forensic Medicine & Toxicology, 14(2):222-234.

Dortet L, Bernabeu S, Gonzalez C, Naas T. Evaluation of the carbapenem detection set™ for the detection and characterization of carbapenemase-producing Enterobacteriaceae. DiagnMicrobiol Infect Dis. 2018;91(3):220–225. doi: 10.1016/j.diagmicrobio.2018.02.012. [PubMed] [CrossRef] [Google Scholar].

Vatansevera, C., Menekseb, S., Dogana, O., Gucera, L.S., Ozera, B., Ergonula,O. and Cana, F. (2020). Co-existence of OXA-48 and NDM-1 in colistin resistant Pseudomonas aeruginosa ST235. Emerg. Microb. Infect., 9: 152-154.

Honda, N.H., Aoki, K., Kamisasanuki, T., Matsuda, N., To, M. and Matsushima, H. (2019). Isolation of three distinct carbapenemase-producing Gram negative bacteria from a Vietnamese medical tourist. J. Infect. Chemother., 25: 811-815.

Yoon, E.J. and Jeong, S.H. (2021). Mobile Carbapenemase Genes in Pseudomonas aeruginosa. Fron. Microbio., 12 (2).

Mutar HM. And Aubaid AH.(2021).Molecular profile of mec A,tst 1,hla,hib,eta,etb,erma and ermab virulence genes in Staphylococcus aureus using RAPD-PCR.Annals of the Romanian Society for cell Biology , 25(4) : 3227-3238.

Al-Azawi,I.H.;AdnanHamadAubaid.andHasson,S.O.(2018).Association between biofilm formation and susceptibility to antibiotics in Staphylococcus lentus isolated from urinary catheterized patients. International Journal of research in pharmaceutical sciences,9(SPL1):1309

Hasson,S.O.; Al-Awady ,M. and Adnan.Hamad Aubaid. (2019).Boosting Antimicrobial Activity of Impenem in combination with Silver Nanoparticles towards S.fonticola and Pantoea sp.Nano Biomedicine and Engineering , 11(2):200-214.

Hasson,S.O.;Adnan.Hamad Aubaid and Al-Azawi,I.H.(2018). Occurrence of Biofilm Formation in Serratia fonticola and Pantoea sp. Isolates among Urinary Catheterized Patients.Nano Medicine and Engineering ,10(3):295-304.

Published

2022-08-26

How to Cite

H. Salih, A. ., H. Aubaid, A. ., & B. Ali , G. . (2022). Molecular analysis of Carbapenem resistant genes in Pseudomonas aeruginosa isolated from Baghdad hospitals. Journal of Contemporary Medical Sciences, 8(4). https://doi.org/10.22317/jcms.v8i4.1257