Comparing COL7A1 gene expression in fibroblast cells of dystrophic epidermolysis bullosa patients with clinical responses to autologous fibroblasts transplantation

Authors

  • Maryam Eslami Department of Genetics, Tehran Medical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
  • Majid Golshanfard Department of Genetics, Tehran Medical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran; Applied Biotechnology Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
  • Amir Bajouri Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
  • Nasser Aghdami Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran.
  • Mahsa Mohammadi Department of Genetics, Tehran Medical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
  • Saeed Shafieian Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
  • Omeed Memarsadeghi Applied Biotechnology Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
  • Alexander Seifalian Nanotechnology & Regenerative Medicine Commercialisation Centre (Ltd), London BioScience Innovation Centre, London, United Kingdom.

DOI:

https://doi.org/10.22317/jcms.v9i1.1314

Keywords:

RDEB, COL7A1, Gene expression, Fibroblast, Transplantation, clinical trial, dermal-epidermal junction

Abstract

Objectives: This clinical research aimed to establish autologous fibroblasts transplantation as a possible treatment for patients with DEJ. The COL7A1 gene expression was also evaluated.

Methods: Six patients (3M and 3F), 4 with no recurrent wounds and 2 with recurrent wounds after surgery, and 15 healthy subjects were included in the study as controls. Quantitative real-time polymerase chain reaction (real-time PCR) analysis of the COL7A1 gene was performed using an oligonucleotide primer pair designed to amplify across the exon/exon junction.

Results: The COL7A1 expression level was down-regulated at exons 26-27, 47-48, 96-97, and 116-117 in all patients′ fibroblasts compared with the healthy controls. However, the expression of the COL7A1 gene in the fibroblasts of the patients with a positive response to the treatment was not significantly changed compared with the patients with the poor response. (ClinicalTrials.gov NCT01908088)

Conclusion: In this study the mRNA expression levels of COL7A1 were significantly less in the patients when compared with healthy controls. However the COL7A1 expression after autologous fibroblasts transplantation was not different between the two groups of patients, and further examination is needed to elucidate the mechanism of the treatment.

References

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Published

2023-02-26

How to Cite

Eslami, M. ., Golshanfard, M. ., Bajouri, A. ., Aghdami, N. ., Mohammadi, M. ., Shafieian, S. ., … Seifalian, A. (2023). Comparing COL7A1 gene expression in fibroblast cells of dystrophic epidermolysis bullosa patients with clinical responses to autologous fibroblasts transplantation. Journal of Contemporary Medical Sciences, 9(1). https://doi.org/10.22317/jcms.v9i1.1314

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