Molecular Immuno-response Effects for Iraqi Lycium barbarm Carotenes upon Normal Human lymphocytes culture
Keywords:Lycium barbarm, Carotenoids, Interleukin-10, TNF-α, CD4
Objectives: The study aimed to investigate the effects of Iraqi Lycium barbarm Carotene on normal human blood lymphocytes at molecular level.
Methods: The normal human blood lymphocytes culture was exposed to Carotene extracted from Iraqi Lycium barbarm to estimate the interleukins expression in treated lymphocytes represented by IL-10 and TNF-α as a moderator of immune response at a molecular scale, and their CD markers changes expression (CD3, CD4&CD8) by flow-cytometer apparatus.
Results: Total carotenes content was 0.33mg/g powdered dried fruit. Potent carotene effect was appeared at concentration of 500µg/ml for treated lymphocytes with increasing CD3 level to get upper level after 2 hours interval, while after 4 hours exposure both CD4 and CD8 levels increased dramatically for lymphocytes treated with 125 µg/ml, and 250µg/ml Lycium carotene. An alteration in cytokines gene expression for IL-10 production in response to carotene treatments at (125,250,500) µg/ml for both intervals, with suppress in tumor necrosis factor (TNF-α) gene expression in relation to β-actin the internal control and the PHA mitogenic agent.
Conclusion: The study concluded that the carotenes extracted from Lycium barbarm fruits seemed to initiate the immune system toward Th2 cell activation rather thanTh1, besides the extract may potentiate IL-10 production.
Ludmila Bogacz-R.; Joanna, H. β-Carotene-properties and production methods.J Food Quality and Safety, 2018; vol.2 (2), page 1–6.
Thomas F. Physicians' Desk Reference for Herbal Medicines(PDR). 2020,Medical Economics company. New Jersey.
-Sahar, Al- Shaban; Estabraq ,Al- Wasiti; and Anam, Al-Salihi. Evaluation of the level of DNA damage and repair in human lymphocytes cultured in the presence of Beta- Carotene using comet assay (single cell gel electrophoresis). raqi J. Comm. Med., Jan. 2016 (1).page 11-14.
Vincenzo, S.; Rosa, R.; Antonio, M. ;Rosa, T. and Monica, R. L. Ciabatta Bread Incorporating Goji (Lycium barbarm L.): A New Potential Functional Product with Impact on Human Health. Foods; 2023, 12, 566, page2-17
Shimal, Y. Abdulhadi ;Raghad, N. G.; and Ghazwan Q. H. Molecular Identifification, Antioxidant Effifficacy of Phenolic Compounds, and Antimicrobial Activity of Beta-Carotene Isolated from Fruiting Bodies of Suillus sp. Karbala International Journal of Modern Science . 2020; Volume 6 Issue 4, page365-374.
Gulay, O.; Deniz, G.; Ayse, K.; Esra, C.; Susana, M.; Basem, Al-Omari ; Daniela, C.; Javad, S.; William, C. A mechanistic updated overview on lycopene as potential anticancer agent. Biomedicine and Pharmacotherapy. 2023; Volume 161, 114428
Marco, A. G.; Ana, E. O.; Cecilia, A. and José, D. L. Chemistry, Occurrence, Properties, Applications, and Encapsulation of Carotenoids. Plants; 2023, 12, 313-345.
Seungjin, N.; Ara, G.; Da, B. K.; Minjeong, P.; Hee, W. J. and Bonglee, K. Role of Antioxidant Natural Products in Management of Infertility: A Review of Their Medicinal Potential. Antioxidants;2020, vol, 9, Page .957-61.
Diana, C.; Eva, F.; Adrian, B. T.; Andrea, B.; Piroska, V.; Maria, P. Kaulmann, A.; andBohn,T. Zeaxanthin-Rich Extract from Super food Lycium barbarm Selectively Modulates the Cellular Adhesion and MAPK Signaling in Melanoma versus Normal Skin Cells In Vitro. Molecules; 2021, 26, 333.
Zhonglian, Y.; Mengqin, X.; Xueping, L.; Rui, W.; Wenjing, L.; Ruirong, Z.; Zhengtao, W.; Li, Y. and Yanhong , S. Characterization of carotenoids in Lycium barbarm fruit by using UPC2-PDA-Q-TOF-MSE couple with deep eutectic solvents extraction and evaluation of their 5α-reductase inhibitory activity. Frontiers in Chemistry; 2022, November, vol.08 page:2-12
Sun, W. ;Mohamad, H. S. and Cheng, Q. Health benefits of wolfberry (Gou Qi Zi, Fructus barbarm L.) on the basis of ancient Chinese herbalism and Western modern medicine. Avicenna J Phytomed. 2021 Mar-Apr; 11(2): 109–119,
Freshney, R.I. Culture of Animal Cell. Wily-Liss, New York; 2021; 8th Edition.
Rafael, F. and Vaclav, V. Advanced Methods in Cellular Immunology, 2000;vol. 3.CRC-Press LLC.U.S.A.
Al-Hili, Z.A.M. A study of cytotoxic, antioxidant, inhibition of angiogenic factors and induction of Cyperusrotundus extracts on several cancer cell lines. 2009; A PhD thesis in Genetic engineering and biotechnology, Baghdad University/Iraq.
- Ahmed Rushdi Abdullah, Zainab Yaseen Mohammed Hasan, Khulood.W.Alsammarraie. Effect of Total Lycium barbarum Carotene on Normal Human Blood Lymphocytes. International Journal of Pharmaceutical Research Oct - Dec 2019 ; Vol 11 Supplementary Issue 1,page:1087-1093.
Zola,H.;Swart, B.; Barry, S.; and Yang,X. CD molecules –human cell differentiation molecules. J Immunol Methods, 2007; vol. 30, pp:319-324.
Zhiyong, X.; Qi, D.; Wenxia, Z.; Yongxiang ,Z. Immune activities of polysaccharides isolated from Lycium barbarm L. What do we know so far? Pharmacology & Therapeutics; Volume 229, January 2022, 107921
Cui, C.; Zhongfu, W.; Guiping, G.; Wenqi, H.; Linjuan, H.; Shuang, S. and Beiwei, Z. Effects of Lycium barbarm Polysaccharides on Immunity and Metabolic Syndrome Associated with the Modulation of Gut Microbiota: A Review. Foods; 2022, 11, 3177.
SAS. Statistical Analysis System, User's Guide. Statistical. Version 9.6th ed. 2018; SAS. Inst. Inc. Cary. N.C. USA
Zainab,Y. M.; Khulood,W.; Subhi,J.H. Quantitative Analysis of Total Polysaccharides and Total Carotene from Lycium barbarm Fruit. International Journal of Modern Biology and Medicine, 2013; 4(2): 64-73.
Doan, T.; Melvold, R.; and Waltenbaugh, C. Lippincott' Illustrated Reviews Immunology. 2021. Wotlers Kluwer Health, Lippincott Williams and Wilkins. London.
Gemala, A.; Fitriyono, A.; and Rafika, E. Critical review on the immunomodulatory activities of carrot’s β-carotene and other bioactive compounds. Journal of Functional Foods 99 (2022) 105303.
- Raksha, A.; Lalit, M. and Navneeta, B. Disease Prevention and Treatment Using β-Carotene: the Ultimate Provitamin A. Revista Brasileira de Farmacognosia (2022) 32:491–501.
- Alireza Milani*, Marzieh Basirnejad*, Sepideh Shahbazi and Azam Bolhassani. Carotenoids: biochemistry, pharmacology and treatment. British Journal of Pharmacology, 2017; vol.174,page: 1290–1324.
-Makarim Q. Al-Lami; Asmaa I. Sail ; Salah M. Al-Chalabi; and Ferial A. Al-Mahdawi. - Study the Effects of Methotrexate with and without Vitamin A on Some Biochemical and Histological Parameters in Male Rabbits. Journal of Biotechnology Research Center,2017; Vol. 11 No.1.
-Amjad A-H. M. ; and Mozahim K. Al-Mallah. Efficient Regeneration of Carrot (Daucus carota L.) plants from cell suspension derived-callus. Journal of Biotechnology Research Center,2015; Vol. 9 No.1.page:58-63.
How to Cite
Copyright (c) 2023 Journal of Contemporary Medical Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.