Isolation, identification and comparative analysis of 16S rRNA of Multidrug-resistant Bacteria Clinically isolated from Al Qassim Region Hospitals in Saudi Arabia
Keywords:Multidrug-resistant, Molecular identification, RNA, Ribosomal, 16S, Polymerase Chain Reaction
Objective: This study concluded that the antibiotic resistance and gene transfer across bacterial strains in the hospital setting are two possible explanations for the observed sequence changes in the target microorganisms.
Methods: The 16S rDNA genes of all isolates were effectively amplified using PCR, and detailed identification results were derived from GenBank databases. The BLAST search resulted in the classification of 28 isolates into five strains. The GC content of bacterial sequences varies greatly between single species.
Results: 16S rDNA was utilized to identify bacterial species from isolates strains results demonstrated that identification of bacterial strains. Sequences varied between and within strains, also, variation are noticed in genomic nucleotide content of isolated and identified strains. Results of the present study demonstrated that the observed heterogeneity in the sequences of our target bacterial strains may be linked to antibiotic resistance and gene transfer between bacteria that evolved as a result of the hospital environment.
Conclusion: This study concluded that antibiotic-resistant bacteria are capable of transferring copies of their DNA encoding a resistance mechanism to other bacteria, even those that are distantly related to them. the observed heterogeneity in the sequences of our target bacterial strains may be linked to antibiotic resistance and gene transfer between bacteria that evolved as a result of the hospital environment.
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