Effect of Glycolipoprotein Biosurfactant from Enterococcus faecium on the viability of Breast cancer cell lines.


  • Dijlah A. Alimeer Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
  • Nibras N. Mahmood Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq.
  • Mohammad M. F. Al-Halbosiy Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq




Anticancer effect, Biosurfactant, Enterococcus faecium, FTIR.


Objective : This study , was research for cytotoxicity of bio-surfactant created by Enterococcus faecium isolated from feces of Iraqi healthy breast-fed infants with  age < 6 months.

Methods :  Cold acetone precipitation was used to the extraction of extracellular Glycolipoprotein biosurfactant and partially purify it. Biosurfactant was then evaluated against two the cell lines, a Breast cancer MCF-7 cell line and a human normal fibroblast cell line NHF), specifically for cell survival and proliferation.  

Results :  At all concentrations  with varying percentage, The viability of the MCF-7 cancer cell line was shown to be reduced with the addition of biosurfactant.; maximum inhibition percentage was 74.2% at a 100 µg/ml concentration, which is lesser than 45.5% cytotoxicity Of NHF healthy fibroblasts cell line.

Conclusions : The findings of this study are highly encouraging in terms of the potential of Glycolipoprotein biosurfactants to treat cancer and encourage additional research with different cell lines.


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How to Cite

A. Alimeer, D. ., N. Mahmood, N. ., & M. F. Al-Halbosiy, M. . (2023). Effect of Glycolipoprotein Biosurfactant from Enterococcus faecium on the viability of Breast cancer cell lines. Journal of Contemporary Medical Sciences, 9(5). https://doi.org/10.22317/jcms.v9i5.1432