Moexipril Improves Renal Ischemia/Reperfusion Injury in Adult Male Rats
DOI:
https://doi.org/10.22317/jcms.v10i1.1477Keywords:
Moexipril, renal ischemia/reperfusion injury (RIRI), inflammatory mediators, oxidative stress, apoptotic factors.Abstract
Objective: The goal of this study is to see if moexipril can protect rats against renal ischemia/reperfusion injury.
Methods: Overall twenty-eight males of rats were divided randomly into four groups (7 rat each group). Sham group: Except for ischemia
induction, these rats underwent IP anesthesia and surgery. Induced group: This group rats were anesthetized and given a midline
laparotomy to induce bilateral renal ischemia for 30 min and 2 hours of reperfusion. DMSO group: Rats received DMSO IP injection 30 min
before ischemia and subjected to 30 min bilateral ischemia and reperfusion for 2 hours, DMSO is a vehicle of moexipril and considered as
control. Moexipril group (pretreated group): moexipril was given in a dose 0.3 mg/kg I.P. injection 30 min before ischemia.
Results: Renal IRI as indicated by a significant increase (P < 0.05) in urea, creatinine, NF-KB P65, IL-1β, and caspase-3 level, while GSH,
SOD, and Bcl-2 levels significantly (P < 0.05) reduced in Renal tissues of rats in the induced group compared to sham group. Moexipril
pretreatment significantly (P < 0.05) ameliorate RIRI as suggested from significant lowering in urea, creatinine, and inflammatory markers
(NF-KB P65 and IL-1β). The renal level of oxidative marker (SOD and GSH) and anti-apoptotic marker Bcl-2 were significant decreased
(P < 0.05) and also significantly increase (P < 0.05) in caspase-3 level with moexipril group in comparison to induced group.
Conclusion: By inhibiting oxidative stress, inflammation, and the apoptotic pathway, moexipril significantly protect from renal ischemia
reperfusion in rats.
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