The Impact of Genetic Polymorphisms in (STIM1 and ORAI1) on Erythropoietin Resistance in Patients with Chronic Renal Failure on Hemodialysis in Iraq
DOI:
https://doi.org/10.22317/jcms.v10i1.1495Keywords:
genetic polymorphisms, STIM1, ORAI1, erythropoietin resistance, hemodialysis, chronic renal failure.Abstract
Objective: This study aims to investigate the correlation between genetic polymorphisms in store-operated calcium channels (SOCs) signaling and resistance to erythropoietin treatment in patients with chronic renal failure (CRF). Anemia is a significant complication of CRF, and understanding the genetic factors contributing to resistance could provide insights into potential mechanisms influencing treatment outcomes. Specifically, the focus is on exploring the relationship between SOCs, a pathway activated by erythropoietin, and resistance to address the challenges associated with anemia in CRF patients.
Methods: In this study, a single nucleotide polymorphism in each STIM1 and ORAI1 gene was selected. Genotyping was done by using allele specific polymerase chain reaction technique and the data was analyzed through the Statistical Package for the Social Sciences. 112 patients with CRF on hemodialysis were enrolled 58.9% of them were men and the mean age was 50.94±13.42.
Results: We found that CC genotype of ORAI1 gene has a higher hemoglobin level, AA genotype of STIM1 has a higher EPO level, the interaction of the two genes showed that CCGG group has a higher Hb level and is considered good responder but represents only 4.5% of the patients
Conclusion: After all the genetic polymorphisms of SOCs genes ORAI1 and STIM1 have no significant impact on erythropoietin resistance in patients with CRF on hemodialysis in Iraq.
References
Kalantar-Zadeh, K., Jafar, T. H., Nitsch, D., Neuen, B. L., & Perkovic, V. (2021). Chronic kidney disease. The lancet, 398(10302), 786-802.
Shiferaw, W. S., Akalu, T. Y., & Aynalem, Y. A. (2020). Risk factors for anemia in patients with chronic renal failure: a systematic review and meta-analysis. Ethiopian journal of health sciences, 30(5).
Kao, C. C., Wong, H. S. C., Wang, Y. J., Chou, W. H., Perwitasari, D. A., Wu, M. S., & Chang, W. C. (2021). The role of genetic polymorphisms in STIM1 and ORAI1 for erythropoietin resistance in patients with renal failure. Medicine, 100(17).
Singh, A. K. (2008). Anemia of chronic kidney disease. Clinical Journal of the American Society of Nephrology, 3(1), 3-6.
Alves, M. T., Vilaça, S. S., Carvalho, M. D. G., Fernandes, A. P., Dusse, L. M. S. A., & Gomes, K. B. (2015). Resistance of dialyzed patients to erythropoietin. Revista brasileira de hematologia e hemoterapia, 37, 190-197.
Ogawa, T., & Nitta, K. (2015). Erythropoiesis-stimulating agent hyporesponsiveness in end-stage renal disease patients. Chronic Kidney Diseases-Recent Advances in Clinical and Basic Research, 185, 76-86.
Elliott, J., Mishler, D., & Agarwal, R. (2009). Hyporesponsiveness to erythropoietin: causes and management. Advances in chronic kidney disease, 16(2), 94-100.
Sharples, E. J., Varagunam, M., Sinnott, P. J., McCloskey, D. J., Raftery, M. J., & Yaqoob, M. M. (2006). The effect of proinflammatory cytokine gene and angiotensin-converting enzyme polymorphisms on erythropoietin requirements in patients on continuous ambulatory peritoneal dialysis. Peritoneal dialysis international, 26(1), 64-68.
Girndt, M., Stenvinkel, P., Ulrich, C., Axelsson, J., Nordfors, L., Barany, P., ... & Köhler, H. (2007). Influence of cytokine gene polymorphisms on erythropoetin dose requirements in chronic haemodialysis patients. Nephrology Dialysis Transplantation, 22(12), 3586-3592.
Jeong, K. H., Lee, T. W., Ihm, C. G., Lee, S. H., & Moon, J. Y. (2008). Polymorphisms in two genes, IL-1B and ACE, are associated with erythropoietin resistance in Korean patients on maintenance hemodialysis. Experimental & Molecular Medicine, 40(2), 161-166.
Al-Radeef, M. Y., Fawzi, H. A., & Allawi, A. A. (2019). ACE gene polymorphism and its association with serum erythropoietin and hemoglobin in Iraqi hemodialysis patients. The Application of Clinical Genetics, 107-112.
Nand, N., Deshmukh, A. R., Joshi, S., & Sachdeva, M. P. (2017). Role of ACE and IL-1β Gene Polymorphisms in Erythropoeitin Hyporesponsive Patients with Chronic Kidney Disease with Anemia. The Journal of the Association of Physicians of India, 65(2), 32-36.
Miller, B. A., Scaduto, R. C., Tillotson, D. L., Botti, J. J., & Cheung, J. Y. (1988). Erythropoietin stimulates a rise in intracellular free calcium concentration in single early human erythroid precursors. The Journal of clinical investigation, 82(1), 309-315.
Cheung, J. Y., Zhang, X. Q., Bokvist, K., Tillotson, D. L., & Miller, B. A. (1997). Modulation of calcium channels in human erythroblasts by erythropoietin. Blood, The Journal of the American Society of Hematology, 89(1), 92-100.
Parekh, A. B., & Putney Jr, J. W. (2005). Store-operated calcium channels. Physiological reviews, 85(2), 757-810.
Lewis, R. S. (2020). Store-operated calcium channels: from function to structure and back again. Cold Spring Harbor perspectives in biology, 12(5), a035055.
Pisoni, R. L., Bragg-Gresham, J. L., Young, E. W., Akizawa, T., Asano, Y., Locatelli, F., ... & Port, F. K. (2004). Anemia management and outcomes from 12 countries in the Dialysis Outcomes and Practice Patterns Study (DOPPS). American Journal of Kidney Diseases, 44(1), 94-111.
Locatelli, F., Pisoni, R. L., Combe, C., Bommer, J., Andreucci, V. E., Piera, L., ... & Held, P. J. (2004). Anaemia in haemodialysis patients of five European countries: association with morbidity and mortality in the Dialysis Outcomes and Practice Patterns Study (DOPPS). Nephrology Dialysis Transplantation, 19(1), 121-132.
Collister, D., Rigatto, C., & Tangri, N. (2017). Anemia management in chronic kidney disease and dialysis: a narrative review. Current opinion in nephrology and hypertension, 26(3), 214-218.
Santos, E. J. F., Dias, R. S. C., Lima, J. F. D. B., Salgado Filho, N., & Miranda dos Santos, A. (2020). Erythropoietin resistance in patients with chronic kidney disease: current perspectives. International journal of nephrology and renovascular disease, 231-237.
Lou, D., Wang, J., & Wang, X. (2020). Single nucleotide polymorphisms in the non-coding region of STIM1 gene are associated with Parkinson disease risk in Chinese Han population. Medicine, 99(9).
Kuo, H. C., Lin, Y. J., Juo, S. H. H., Hsu, Y. W., Chen, W. C., Yang, K. D., ... & Chang, W. C. (2011). Lack of association between ORAI1/CRACM1 gene polymorphisms and Kawasaki disease in the Taiwanese children. Journal of Clinical Immunology, 31, 650-655.
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