Improved Exopolysaccharide Production from Lacticaseibacillus Paracasei SH6 Using Mutagen EMS

Authors

  • Marwa R. Ali Department of Food Science, Faculty of Agriculture, Cairo University, Giza, Egypt.
  • Zaid Akram Thabit Environmental Biotech Department, Al-Nahrain University, Baghdad, Iraq.
  • Shatha A. Allaith Field Crops Department, Faculty of Agriculture, Karbala University, Karbala, Iraq.
  • Hanan S. Ebrahim Plant Biotechnology Department, National Research Centre (NRC), Cairo, Egypt.
  • Ahmed A. Radwan Genetics and Cytology Department, National Research Centre (NRC), Cairo, Egypt.
  • Basant A. Ali Microbial Chemistry Department, National Research Centre (NRC), Cairo, Egypt.
  • Ahmed M. M. Gabr Plant Biotechnology Department, National Research Centre (NRC), Cairo, Egypt.

DOI:

https://doi.org/10.22317/jcms.v11i1.1706

Keywords:

Lacticaseibacillus paracasei; Exopolysaccharide (EPS); Ethyl methanesulfonate (EMS); Dextran.

Abstract

Objective: This study aims to enhance exopolysaccharide (EPS) production from a local isolate of Lacticaseibacillus paracasei through ethyl methanesulfonate (EMS)-induced chemical mutagenesis.

Methods: Two isolates, L. paracasei SH5 and SH6, were obtained from fermented beverages (boza and cider) and initially screened for EPS production. The SH6 isolate was subjected to EMS-induced mutation. Antibiotic-resistant mutants were selected from the inhibition zones surrounding antibiotic discs. EPS production was assessed through total soluble carbohydrate content analysis, HPLC calculations, and gene expression studies.

Results: Among the selected mutants, M8 exhibited the highest EPS production, with a yield of 566 mg/100 ml compared to the wild-type isolate, which produced 362 mg/100 ml. Other mutants (M1, M4, and M7) also showed increased EPS levels, reaching 411, 488, and 457 mg/100 ml, respectively. Mutant M8 demonstrated a 1.5-fold increase in EPS production compared to the wild type.

Conclusion: The findings confirm that EMS-induced mutagenesis effectively enhances EPS production in L. paracasei. This improvement has significant implications for biotechnological applications, particularly in the pharmaceutical and food industries.

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Published

2025-02-26

How to Cite

Ali, M. R., Thabit , Z. A., Allaith , S. A., Ebrahim , H. S., Radwan , A. A., Ali , B. A., & Gabr, A. M. M. (2025). Improved Exopolysaccharide Production from Lacticaseibacillus Paracasei SH6 Using Mutagen EMS. Journal of Contemporary Medical Sciences, 11(1). https://doi.org/10.22317/jcms.v11i1.1706

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Vol. 11 No. 1 (2025): January-February

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