Association of Cytomegalovirus load, miR-135a-5p Expression, and C- Reactive Protein in Women with Recurrent Pregnancy Loss: A Case –Control Study
DOI:
https://doi.org/10.22317/jcms.v11i5.2030Keywords:
Abortion, Habitual, Cytomegalovirus, C-Reactive Protein, MicroRNAs, Polymerase Chain ReactionAbstract
Objective: To assess the association between CMV viral load, serum CRP levels, and miR-135a-5p expression in women with RPL compared to healthy pregnant controls.
Methods: A case –control study was conducted involving 60 women with ≥ 3 first- trimester losses and 30 healthy pregnant women. Blood samples were analyzed for CMV DNA via quantitative polymerase chain reaction (PCR), miR-135a-5p expression via RT- qPCR, and CRP levels via ELISA. Statistical correlations were evaluated.
Result: CMV DNA was detected in 81.7% of RPL patients vs. 23.3% of controls (p < 0.0001). Mean CMV load, CRP levels, and miR-135a-5p expression were significantly higher in the RPL group. Strong positive correlation was observed among the three biomarkers (r ˃ 0.6, p < 0.0001).
Conclusion: Elevated CMV load, CRP levels, and miR-135a-5p expression are significantly associated with RPL. These markers may reflect a common pathogenic pathway involving infection-induced inflammation and epigenetic regulation.
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