An electrochemical sensing platform for sensitive detection DNA methylation using Fe3O4/TMC/Au nanocomposite and poly(l-arginine)/reduced graphene oxide modified screen-printed carbon electrode


  • Leila Syedmoradi Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Hassan Hajghassem Department of Microelectromechanical systems, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.
  • Gholamreza Tavoosidana Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Seyed Mahdi Rezayat Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Reza Faridi-Majidi Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Kobra Omidfar Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran; Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran.



Fe3O4/TMC/Au nanoparticle, polyarginine, DNA methylation, SEPT9, anti-5-methylcytosine antibody


Objectives: In this study, a simple electrochemical nano-genosensor has been developed for the rapid and sensitive detection of methylated SEPT9 DNA as a useful biomarker for early colorectal cancer detection or screening.

Methods: The process consists of three main steps: (i) the surface modification of screen-printed carbon electrode (SPCEs) with a poly(l-Arg)/RGO composite film followed by immobilizing anti-5-methylcytosine antibody (ii) preparation of probe-modified Fe3O4/TMC/Au nanocomposites for the hybridization with complementary DNA sequences, (iii) capturing methylated DNA target by antibody-modified SPCEs and subsequent electrochemical detection through redox peak currents of gold nanoparticles which generated a concentration-dependent response.

Results: The surface modification of the electrode and hybridization with the methylated target were confirmed by cyclic voltammetry (CV) method and differential pulse voltammetry (DPV) was employ for quantitative evaluation of methylated target DNA.

Conclusion: The assay showed a wide linear range from 0.01 pM to 1000 pM with a low detection limit of 0.01pM.


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How to Cite

Syedmoradi, L., Hajghassem, H., Tavoosidana, G., Rezayat, S. M., Faridi-Majidi, R., & Omidfar, K. (2018). An electrochemical sensing platform for sensitive detection DNA methylation using Fe3O4/TMC/Au nanocomposite and poly(l-arginine)/reduced graphene oxide modified screen-printed carbon electrode. Journal of Contemporary Medical Sciences, 4(4), 216–221.