An electrochemical sensing platform for sensitive detection DNA methylation using Fe3O4/TMC/Au nanocomposite and poly(l-arginine)/reduced graphene oxide modified screen-printed carbon electrode
DOI:
https://doi.org/10.22317/jcms.v4i4.424Keywords:
Fe3O4/TMC/Au nanoparticle, polyarginine, DNA methylation, SEPT9, anti-5-methylcytosine antibodyAbstract
Objectives: In this study, a simple electrochemical nano-genosensor has been developed for the rapid and sensitive detection of methylated SEPT9 DNA as a useful biomarker for early colorectal cancer detection or screening.
Methods: The process consists of three main steps: (i) the surface modification of screen-printed carbon electrode (SPCEs) with a poly(l-Arg)/RGO composite film followed by immobilizing anti-5-methylcytosine antibody (ii) preparation of probe-modified Fe3O4/TMC/Au nanocomposites for the hybridization with complementary DNA sequences, (iii) capturing methylated DNA target by antibody-modified SPCEs and subsequent electrochemical detection through redox peak currents of gold nanoparticles which generated a concentration-dependent response.
Results: The surface modification of the electrode and hybridization with the methylated target were confirmed by cyclic voltammetry (CV) method and differential pulse voltammetry (DPV) was employ for quantitative evaluation of methylated target DNA.
Conclusion: The assay showed a wide linear range from 0.01 pM to 1000 pM with a low detection limit of 0.01pM.
References
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27. Shirazi H, Daneshpour M, Kashanian S, Omidfar K. Synthesis, characterization and in vitro biocompatibility study of Au/TMC/Fe3O4 nanocomposites as a promising, nontoxic system for biomedical applications. Beilstein J Nanotechnol. 2015;6:1677–1689.
28. Daneshpour M, Omidfar K, Ghanbarian H. A novel electrochemical nanobiosensor for the ultrasensitive and specific detection of femtomolarlevel gastric cancer biomarker miRNA-106a. Beilstein J Nanotechnol. 2016;7:2023–2036.
29. Daneshpour M, Karimi B, Omidfar K. Simultaneous detection of gastric cancer-involved miR-106a and let-7a through a dual-signal-marked electrochemical nanobiosensor. Biosens Bioelectron. 2018;109:197–205.
30. Mousavi MF, Amiri M, Noori A, Khoshfetrat SM. A prostate specific antigen immunosensor based on biotinylated-antibody/cyclodextrin inclusion complex: fabrication and electrochemical studies. Electroanalysis. 2017;29:2818–2831.
31. Omidfar K, Rasaee MJ, Zaraee AB, Amir MP, Rahbarizadeh F. Stabilization of penicillinase-hapten conjugate for enzyme immunoassay. J Immunoassay Immunochem. 2002;23:385–398.
32. Shirazi H, Ahmadi A, Darzianiazizi M, Kashanian S, Kashanian S, Omidfar K. Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection. IET Nanobiotechnol. 2016;10:20–27.
2. Paska AV, Hudler P. Aberrant methylation patterns in cancer: a clinical view. Biochem Med (Zagreb). 2015;25:161–176.
3. Feinberg AP, Vogelstein B. Hypomethylation distinguishes genes of some human cancers from their normal counterparts. Nature. 1983;301:89–92.
4. Mikeska T, Craig JM. DNA methylation biomarkers: cancer and beyond. Genes (Basel). 2014;5:821–864.
5. Mikeska T, Bock C, Do H, Dobrovic A. DNA methylation biomarkers in cancer: progress towards clinical implementation. Expert Rev Mol Diagn. 2012;12:473–487.
6. Warton K, Samimi G. Methylation of cell-free circulating DNA in the diagnosis of cancer. Front Mol Biosci. 2015;2:13.
7. Li Y, Song L, Gong Y, He B. Detection of colorectal cancer by DNA methylation biomarker SEPT9: past, present and future. Biomark Med. 2014;8:755–769.
8. Song L, Jia J, Peng X, Xiao W, Li Y. The performance of the SEPT9 gene methylation assay and a comparison with other CRC screening tests: a meta-analysis. Sci Rep. 2017;7:3032.
9. Nian J, Sun X, Ming S, Yan C, Ma Y, Feng Y, et al. Diagnostic accuracy of methylated SEPT9 for blood-based colorectal cancer detection: a systematic review and meta-analysis. Clin Transl Gastroenterol. 2017;8:e216.
10. Kurdyukov S, Bullock M. DNA methylation analysis: choosing the right method. Biology (Basel). 2016;5. pii: E3.
11. Noehammer C, Pulverer W, Hassler MR, Hofner M, Wielscher M, Vierlinger K, et al. Strategies for validation and testing of DNA methylation biomarkers. Epigenomics. 2014;6:603–622.
12. Syedmoradi L, Esmaeili F, Norton ML. Towards DNA methylation detection using biosensors. Analyst. 2016;141:5922–5943.
13. Taleat Z, Mathwig K, Sudhölter EJR, Rassaei L. Detection strategies for methylated and hypermethylated DNA. TrAC, Trends Anal Chem. 2015;66:80–89.
14. Krejcova L, Richtera L, Hynek D, Labuda J, Adam V. Current trends in electrochemical sensing and biosensing of DNA methylation. Biosens Bioelectron. 2017;97:384–399.
15. Hossain T, Mahmudunnabi G, Masud MK, Islam MN, Ooi L, Konstantinov K, et al. Electrochemical biosensing strategies for DNA methylation analysis. Biosens Bioelectron. 2017;94:63–73.
16. Daneshpour M, Moradi LS, Izadi P, Omidfar K. Femtomolar level detection of RASSF1A tumor suppressor gene methylation by electrochemical nano-genosensor based on Fe3O4/TMC/Au nanocomposite and PT-modified electrode. Biosens Bioelectron. 2016;77:1095–1103.
17. Rahman MM, Li XB, Lopa NS, Ahn SJ, Lee JJ. Electrochemical DNA hybridization sensors based on conducting polymers. Sensors (Basel). 2015;15:3801–3829.
18. Liu Z, Forsyth H, Khaper N, Chen A. Sensitive electrochemical detection of nitric oxide based on AuPt and reduced graphene oxide nanocomposites. Analyst. 2016;141:4074–4083.
19. Li D, Yang XL, Xiao BL, Geng FY, Hong J, Sheibani N, et al. Detection of guanine and adenine using an aminated reduced graphene oxide functional membrane-modified glassy carbon electrode. Sensors. 2017;17. pii: E1652.
20. Kanyong P, Rawlinson S, Davis J. A voltammetric sensor based on chemically reduced graphene oxide-modified screen-printed carbon electrode for the simultaneous analysis of uric acid, ascorbic acid and dopamine. Chemosensors. 2016;4:25.
21. Haque AM, Park H, Sung D, Jon S, Choi SY, Kim K. An electrochemically reduced graphene oxide-based electrochemical immunosensing platform for ultrasensitive antigen detection. Anal Chem. 2012;84:1871–1878.
22. Jian JM, Liu YY, Zhang YL, Guo XS, Cai Q. Fast and sensitive detection of Pb2+ in foods using disposable screen-printed electrode modified by reduced graphene oxide. Sensors (Basel). 2013;13:13063–13075.
23. Khoshfetrat SM, Mehrgardi MA. Amplified electrochemical genotyping of single-nucleotide polymorphisms using a graphene–gold nanoparticles modified glassy carbon platform. RSC Advances. 2015;5:29285–29293.
24. Devadas B, Cheemalapati S, Chen S-M, Ali MA, Al-Hemaid FM. Highly sensing graphene oxide/poly-arginine-modified electrode for the simultaneous electrochemical determination of buspirone, isoniazid and pyrazinamide drugs. Ionics. 2015;21:547–555.
25. Li Y, Feng X, Zhao L, Pu Q. On-surface formation of polyarginine/reduced graphene oxide film and its application in measuring puerarin in healthcare products. Int. J. Electrochem. Sci. 2018;13:3948–3957.
26. Zhang F, Wang Z, Zhang Y, Zheng Z, Wang C, Du Y, et al. Simultaneous electrochemical determination of uric acid, xanthine and hypoxanthine based on poly (L-arginine)/graphene composite film modified electrode. Talanta. 2012;93:320–325.
27. Shirazi H, Daneshpour M, Kashanian S, Omidfar K. Synthesis, characterization and in vitro biocompatibility study of Au/TMC/Fe3O4 nanocomposites as a promising, nontoxic system for biomedical applications. Beilstein J Nanotechnol. 2015;6:1677–1689.
28. Daneshpour M, Omidfar K, Ghanbarian H. A novel electrochemical nanobiosensor for the ultrasensitive and specific detection of femtomolarlevel gastric cancer biomarker miRNA-106a. Beilstein J Nanotechnol. 2016;7:2023–2036.
29. Daneshpour M, Karimi B, Omidfar K. Simultaneous detection of gastric cancer-involved miR-106a and let-7a through a dual-signal-marked electrochemical nanobiosensor. Biosens Bioelectron. 2018;109:197–205.
30. Mousavi MF, Amiri M, Noori A, Khoshfetrat SM. A prostate specific antigen immunosensor based on biotinylated-antibody/cyclodextrin inclusion complex: fabrication and electrochemical studies. Electroanalysis. 2017;29:2818–2831.
31. Omidfar K, Rasaee MJ, Zaraee AB, Amir MP, Rahbarizadeh F. Stabilization of penicillinase-hapten conjugate for enzyme immunoassay. J Immunoassay Immunochem. 2002;23:385–398.
32. Shirazi H, Ahmadi A, Darzianiazizi M, Kashanian S, Kashanian S, Omidfar K. Signal amplification strategy using gold/N-trimethyl chitosan/iron oxide magnetic composite nanoparticles as a tracer tag for high-sensitive electrochemical detection. IET Nanobiotechnol. 2016;10:20–27.
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Published
2018-12-26
How to Cite
Syedmoradi, L., Hajghassem, H., Tavoosidana, G., Rezayat, S. M., Faridi-Majidi, R., & Omidfar, K. (2018). An electrochemical sensing platform for sensitive detection DNA methylation using Fe3O4/TMC/Au nanocomposite and poly(l-arginine)/reduced graphene oxide modified screen-printed carbon electrode. Journal of Contemporary Medical Sciences, 4(4), 216–221. https://doi.org/10.22317/jcms.v4i4.424
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