Antioxidative and anti-inflammatory effects of Cichorium intybus L. seed extract in ischemia/reperfusion injury model of rat spinal cord


  • Neda Ghaffari Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Gholamreza Hassanzadeh Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Azin Nowrouzi Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Morteza Gholaminejhad Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Tahmineh Mokhtari Nervous System stem cells Research Center, Semnan University of Medical Sciences, Semnan, Iran
  • Rahimeh Seifali Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Ibrahim Mohammed Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Mohammad Akbari Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran



Cichorium intybus L., spinal cord ischemia/reperfusion, inflammation, oxidative stress


Objectives: The antioxidant and anti-inflammatory effects of aqueous extract of chicory seed (CSE, Cichorium intybus L. seed) following spinal cord ischemia/reperfusion (SCI/R) injury in rat model were evaluated.

Methods: In this study 36 male Wistar rats were randomly divided to six groups:  control (Co), sham (Sh), CSE, SCI/R, CSE+SCI/R (7 days pretreatment with CSE group+inducing I/R injury), SCI/R +CSE (induced I/R injury group+3 days treatment with CSE). SCI/R injury was induced by creating a longitudinal incision on the midline of abdominal region and clamping the aorta just below renal artery for 30 minutes. After 3 days, SC was removed and used for evaluation of antioxidant enzymes (including Superoxide dismutase [SOD] and catalase [CAT]), oxidative stress markers (malondialdehyde [MDA]), inflammatory factors (IL1β, IL18 & TNFα) and histopathological changes. Before sacrificing the animals, the motional score were assessed.

Results: Our results demonstrated that, in the SCI/R group, the mean  levels of SOD, and CAT were significantly decreased (P<0.05), while the mean level of MDA was significantly increased (P<0.05) in comparison to Co and Sh groups. Also, the mean levels of SOD and CAT in the treatment group were higher than the SCI/R group (P<0.05), while, the mean MDA content in the treatment group was significantly less than the SCI/R group (P <0.05). In addition, comparison between SCI/R and treatment groups demonstrated a significant decrease in tissue damage in the treatment group.

Conclusions: Our study demonstrated that, the neuroprotective effects of aqueous extract of Cichorium intybus L. seed on SCI/R injury in rat by anti-oxidative and anti-inflammatory activities. Additionally, comparing the treatment and pretreatment groups shows that the pretreatment usage of the extract is more effective than treatment group.


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How to Cite

Ghaffari, N., Hassanzadeh, G., Nowrouzi, A., Gholaminejhad, M., Mokhtari, T., Seifali, R., Mohammed, I., & Akbari, M. (2018). Antioxidative and anti-inflammatory effects of Cichorium intybus L. seed extract in ischemia/reperfusion injury model of rat spinal cord. Journal of Contemporary Medical Sciences, 4(4), 195–201.




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