Fibrin-collagen hydrogel as a scaffold for dermoepidermal skin substitute, preparation and characterization
DOI:
https://doi.org/10.22317/jcms.v5i1.519Keywords:
Tissue engineering, Fibrin-collagen, Hydrogel, Skin substituteAbstract
Objective: Bioengineered skin substitutes were created to address wound healing problems. Skin substitutes contains live human cells seeded onto a matrix to provide cytokine, growth factor and other proteins from ECM required to decrease healing time. These products are classified based on their durability, the cells seeded on them and their originality. In this study, we aimed to investigate fibrin-collagen hydrogel as a new scaffold to design a bilayer temporary skin equivalent.
Methods: Fibrin gel was prepared by crosslinking fibrinogen with thrombin and mixing it with collagen type 1. Human fibroblasts and keratinocytes were isolated from skin biopsies of healthy donors and foreskin and the cells were seeded onto 3D hydrogel layer by layer. Morphological assessment, histological analysis, immunocytochemistry were performed to characterize the scaffold properties.
Result: The results of scaffold characterization demonstrated good porosity, cell viability and biocompatibility of the scaffold.
Conclusion: Fibrin-collagen as a natural material in organotypic cell culture modeling demonstrates that hydrogel scaffolds can be properly designed to generate bilayer or composite temporary skin grafts.
References
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27. Schoop VM, Mirancea N, Fusenig NE. Epidermal organization and differentiation of HaCaT keratinocytes in organotypic coculture with human dermal fibroblasts. The Journal of investigative dermatology. 1999;112(3):343-53.
28. Pontiggia L, Biedermann T, Meuli M, Widmer D, Bottcher-Haberzeth S, Schiestl C, et al. Markers to evaluate the quality and self-renewing potential of engineered human skin substitutes in vitro and after transplantation. The Journal of investigative dermatology. 2009;129(2):480-90.
2. Nilforoushzadeh MA, Sisakht MM, Seifalian AM, Amirkhani MA, Banafshe HR, Verdi J, et al. Regenerative Medicine Applications in Wound Care. Current stem cell research & therapy. 2017;12(8):658-74.
3. Alrubaiy L, Al-Rubaiy KK. Skin substitutes: a brief review of types and clinical applications. Oman medical journal. 2009;24(1):4-6.
4. Halim AS, Khoo TL, Mohd Yussof SJ. Biologic and synthetic skin substitutes: An overview. Indian journal of plastic surgery : official publication of the Association of Plastic Surgeons of India. 2010;43(Suppl):S23-8.
5. Kamoun EA, Kenawy ES, Chen X. A review on polymeric hydrogel membranes for wound dressing applications: PVA-based hydrogel dressings. Journal of advanced research. 2017;8(3):217-33.
6. Akhtar MF, Hanif M, Ranjha NM. Methods of synthesis of hydrogels ... A review. Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society. 2016;24(5):554-9.
7. Madaghiele M, Demitri C, Sannino A, Ambrosio L. Polymeric hydrogels for burn wound care: Advanced skin wound dressings and regenerative templates. Burns & trauma. 2014;2(4):153-61.
8. Parenteau NL, Bilbo P, Nolte CJ, Mason VS, Rosenberg M. The organotypic culture of human skin keratinocytes and fibroblasts to achieve form and function. Cytotechnology. 1992;9(1-3):163-71.
9. Klar AS, Guven S, Zimoch J, Zapiorkowska NA, Biedermann T, Bottcher-Haberzeth S, et al. Characterization of vasculogenic potential of human adipose-derived endothelial cells in a three-dimensional vascularized skin substitute. Pediatric surgery international. 2016;32(1):17-27.
10. Klar AS, Guven S, Biedermann T, Luginbuhl J, Bottcher-Haberzeth S, Meuli-Simmen C, et al. Tissue-engineered dermo-epidermal skin grafts prevascularized with adipose-derived cells. Biomaterials. 2014;35(19):5065-78.
11. Simoni RC, Lemes GF, Fialho S, Goncalves OH, Gozzo AM, Chiaradia V, et al. Effect of drying method on mechanical, thermal and water absorption properties of enzymatically crosslinked gelatin hydrogels. Anais da Academia Brasileira de Ciencias. 2017;89(1 Suppl 0):745-55.
12. H.A. C. Masson's Trichrome Stain in the Evaluation of Renal Biopsies. AJCP. 1975;65:631-43.
13. Ahmed TA, Dare EV, Hincke M. Fibrin: a versatile scaffold for tissue engineering applications. Tissue engineering Part B, Reviews. 2008;14(2):199-215.
14. Gibs I. JH. Review: synthetic polymer hydrogels for biomedical applications. Chem Chem Tech. 2010;4(4):297-304.
15. Bracaglia LG, Messina M, Winston S, Kuo CY, Lerman M, Fisher JP. 3D Printed Pericardium Hydrogels To Promote Wound Healing in Vascular Applications. Biomacromolecules. 2017;18(11):3802-11.
16. Hu Y, Zhang Z, Li Y, Ding X, Li D, Shen C, et al. Dual-Crosslinked Amorphous Polysaccharide Hydrogels Based on Chitosan/Alginate for Wound Healing Applications. Macromolecular rapid communications. 2018;39(20):e1800069.
17. Annabi N, Rana D, Shirzaei Sani E, Portillo-Lara R, Gifford JL, Fares MM, et al. Engineering a sprayable and elastic hydrogel adhesive with antimicrobial properties for wound healing. Biomaterials. 2017;139:229-43.
18. El-Sherbiny IM, Yacoub MH. Hydrogel scaffolds for tissue engineering: Progress and challenges. Global cardiology science & practice. 2013;2013(3):316-42.
19. Shin H, Jo S, Mikos AG. Biomimetic materials for tissue engineering. Biomaterials. 2003;24(24):4353-64.
20. Boyan BD, Hummert TW, Dean DD, Schwartz Z. Role of material surfaces in regulating bone and cartilage cell response. Biomaterials. 1996;17(2):137-46.
21. Li Y, Meng H, Liu Y, Lee BP. Fibrin gel as an injectable biodegradable scaffold and cell carrier for tissue engineering. TheScientificWorldJournal. 2015;2015:685690.
22. Christman KL, Fok HH, Sievers RE, Fang Q, Lee RJ. Fibrin glue alone and skeletal myoblasts in a fibrin scaffold preserve cardiac function after myocardial infarction. Tissue engineering. 2004;10(3-4):403-9.
23. Cakmak O, Babakurban ST, Akkuzu HG, Bilgi S, Ovali E, Kongur M, et al. Injectable tissue-engineered cartilage using commercially available fibrin glue. The Laryngoscope. 2013;123(12):2986-92.
24. Litvinov RI, Weisel JW. Fibrin mechanical properties and their structural origins. Matrix biology : journal of the International Society for Matrix Biology. 2017;60-61:110-23.
25. Kretzschmar K, Watt FM. Markers of epidermal stem cell subpopulations in adult mammalian skin. Cold Spring Harbor perspectives in medicine. 2014;4(10).
26. Watt FM. Epidermal stem cells: markers, patterning and the control of stem cell fate. Philosophical transactions of the Royal Society of London Series B, Biological sciences. 1998;353(1370):831-7.
27. Schoop VM, Mirancea N, Fusenig NE. Epidermal organization and differentiation of HaCaT keratinocytes in organotypic coculture with human dermal fibroblasts. The Journal of investigative dermatology. 1999;112(3):343-53.
28. Pontiggia L, Biedermann T, Meuli M, Widmer D, Bottcher-Haberzeth S, Schiestl C, et al. Markers to evaluate the quality and self-renewing potential of engineered human skin substitutes in vitro and after transplantation. The Journal of investigative dermatology. 2009;129(2):480-90.
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Published
2019-02-26
How to Cite
Mollapour Sisakht, M., Nilforoushzadeh, M. A., Verdi, J., Banafshe, H. R., Safaei Naraghi, Z., & Mortazavi-Tabatabaei, S. A. (2019). Fibrin-collagen hydrogel as a scaffold for dermoepidermal skin substitute, preparation and characterization. Journal of Contemporary Medical Sciences, 5(1), 8–13. https://doi.org/10.22317/jcms.v5i1.519
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