The Biochemical parameters in venous blood sampling by direct venipuncture and peripheral intravenous catheter


  • Fatemeh Jaafaraghaei Nursing and Midwifery Faculty, Guilan University of Medical Sciences, Rasht, Iran.
  • Monireh Aghajany-Nasab Department of Biochemistry, Molecule and Cell Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
  • Mahsa Aghaei Nursing and Midwifery Faculty, Guilan University of Medical Sciences, Rasht, Iran.
  • Nazila Javadi pashaki Social Determinants of Health Research Center, Nursing and Midwifery Faculty, Guilan University of Medical Sciences, Rasht, Iran.
  • Ehsan Kazemnejhad Leili Nursing and Midwifery Faculty, Guilan University of Medical Sciences, Rasht, Iran.



Biochemical values, Peripheral Catheterization, Blood Specimen Collection


Objective: The aim of this study was to compare the results of some biochemical values in venous blood samples obtained by direct venipuncture (DV) and peripheral venous catheter (PVC).

Methods: In this cross-sectional study, 78 hospitalized patients in different wards of Poor-Sina Medical and Educational Center of Rasht in 2017 were divided into three equal groups, including patients who were treated with normal saline and dextrose saline and those who did not receive this solution. Two separate blood samples were obtained from each patient, one from PVC and the other from DV. The levels of sodium, potassium, creatinine and blood urea nitrogen (BUN) were measured and compared.

Results: Difference of PVC with DV in BUN was generally 0.32±3.18 mg/dl; creatinine, sodium and potassium were 0.02±0.12 mg/dl, 1.00±2.49 mEq/L and 0.15±0.48 mEq/L respectively. There were no statistically significant differences between the two methods in relation to BUN and creatinine (P = 0.377 and P = 0.149, respectively), but significant differences in levels of sodium and potassium were observed between the two blood sampling methods (P = 0.001 and P = 0.008, respectively).

Conclusion: According to the results of this study and other studies, PVC can be used to measure BUN and creatinine, not so for sodium and potassium. For other parameters, further investigation is needed.


1. Bernhofer EI, St Marie B, Bena JF. A New Clinical Pain Knowledge Test for Nurses: Development and Psychometric Evaluation. Pain Manag Nurs. 2017 Aug; 18(4):224-233.
2. Yilmaz Kurt F, Aytekin Ozdemir A, Atay S. The Effects of Two Methods on Venipuncture Pain in Children: Procedural Restraint and Cognitive-Behavioral Intervention Package. Pain Manag Nurs. 2019 Oct 15. pii: S1524-9042(18)30213-3.
3. Cadacio C, Nachamkin I. A Novel Needle-Free Blood Draw Device for Sample Collection From Short Peripheral Catheters. J Infus Nurs. 2017; 40(3):156–162.
4. Aliasgharpour M, Jafari R, Jalalinia S F, Jalal Madani S, Tabari F, Kazemnegad Lili A. Comparing Blood Values Sampled From Venipuncture and Continuous Infusion Catheter. Crit Care Nurs J. 2016; 9 (3): 1-5.
5. Ortells-Abuye N, Busquets-Puigdevall T, Díaz-Bergara M, Paguina-Marcos M, Sánchez-Pérez I. A cross-sectional study to compare two blood collection methods: direct venous puncture and peripheral venous catheter. BMJ Open. 2014; 4(2): e004250.
6. Kennedy RM, Luhmann J, Zempsky WT. Clinical implications of unmanaged needle-insertion pain and distress in children. Pediatrics. 2008 Nov; 122 Suppl 3:S130-3.
7. Gholipour Baradari A, Zargar N, Aarabi M, Koohsari E, Emami Zeydi A. Comparison of Hematologic and Biochemical Test Results in Blood Samples Obtained by Venipuncture and Peripheral Intravenous Catheter. J Mazandaran Univ Med Sci. 2016; 26 (139):66-72 (Persian).
8. Amini M, Vaseie M, Ansari I. The evaluation of nosocomial urinary tract infections and antimicrobial resistance in ICU patients, Tehran, Iran, 2012-2016. Acta Medica Mediterranea, 2017, 33: 945-52
9. Asheghan M, Khatibi A, Holisaz MT. Paresthesia and forearm pain after phlebotomy due to medial antebrachial cutaneous nerve injury. J Brachial Plex Peripher Nerve Inj. 2011; 6:5.
10. Hambleton VL, Gómez IA, Andreu FA. Venipuncture versus peripheral catheter: do infusions alter laboratory results? J Emerg Nurs. 2014 Jan; 40(1):20-6.
11. Yazdankhahfard M, Taghizadeganzadeh M, Farzaneh M, Mirzaei K. Comparsion of Biochemical Laboratory Values Obtained by Means of Routine Method of Venipuncture Versus Peripheral Intravenous Infusion Line after Administration of Fluids. Armaghane danesh. 2015; 19 (12) :1021-1028 (Persian).
12. Braniff H, DeCarlo A, Haskamp AC, Broome ME. Pediatric blood sample collection from a pre-existing peripheral intravenous (PIV) catheter. J Pediatr Nurs. 2014 Sep-Oct;29(5):451-6.
13. Mofrad ZP, Shafiee S, Mahmoodi N, Jahantigh M, Samadzadeh H. Comparison of the effect of direct venipuncture and venous catheter blood sampling methods on the biochemical results of patients hospitalized in CCU. Medical-Surgical Nursing Journal. 2016;5(1):11-5.
14. Cicolini G, Manzoli L, Simonetti V, Flacco ME, Comparcini D, Capasso L, et al. Phlebitis risk varies by peripheral venous catheter site and increases after 96 hours: a large multicentre prospective study. J Adv Nurs. 2014 Nov; 70(11):2539-49.
15. Aghaei-Hashjin M, Javadi-Pashaki N, Kazemnezhad-Leyli E, Aghajani-Nasab M. Comparison of the results of blood sugar between direct blood sampling and peripheral catheter in hospitalized patients. J Biochem Tech. 2018; Special Issue (2): 189-195
16. Watson KR, O’kell RT, Joyce JT. Data regarding blood drawing sites in patients receiving intravenous fluids. Am J Clin Pathol. 1983 Jan; 79(1):119-21.
17. Zlotowski SJ, Kupas DF, Wood GC. Comparison of laboratory values obtained by means of routine venipuncture versus peripheral intravenous catheter after a normal saline solution bolus. Ann Emerg Med. 2001 Nov; 38(5):497-504.
18. Rezaei K, Kohestani H, Zand S. Comparison of Biochemistry Values Obtained by Venipuncture and Saline Lock after Intermittent Administration of Fluids and drugs. J Arak Uni Med Sci. 2009; 12 (1):49-56 (Persian).
19. Baker RB, Summer SS, Lawrence M, Shova A, McGraw CA, Khoury J. Determining optimal waste volume from an intravenous catheter. J Infus Nurs. 2013;36(2):92–96




How to Cite

Jaafaraghaei, F., Aghajany-Nasab, M., Aghaei, M., Javadi pashaki, N., & Kazemnejhad Leili, E. (2020). The Biochemical parameters in venous blood sampling by direct venipuncture and peripheral intravenous catheter. Journal of Contemporary Medical Sciences, 6(5), 208–212.