Comparison between manual procedure and automated for determinant of WBCs and PCV in maternity and labor hospital in Karbala city

Abstract

Objectives This study was designed to determine white blood cells (WBCs) and hematocrit (packed cell volume PCV) by manual procedure and comprised with an automated method in the maternity and labor hospital in Karbala city.
Methods During the period of comparative cross sectional study, 52 cases of blood sample were collected into tri-potassium ethylenediamine tetra-acetic acid (K3EDTA) from pregnant women admitted to hospital during February to April 2016 for the analysis of PCV and WBCs by Sysmex XP-300TM in the heamatological department laboratory performance manual method in the same time. All data analyzed by Pearson correlation coefficient as well as using 2 Mean & Standard deviation for both methods.
Results For two hematologic parameter test, the correlation coefficient was (R = 0.75) for hematocrit thus improved strong correlation between two procedure manual and automated to evaluate WBCs (R = 0.94), which was significantly different (P < 0.001) for two parameter methods.
Conclusion From the results of our research, it can be concluded that the automated analyzer (WBCs) and (Hb) were well correlated with manual standard method for two hematologic parameter. So health care workers in the laboratory can save performance time for hematologic parameter in automated analyzer.