Evaluation of T-Bet immunostaining in the counting of intraepithelial lymphocytes in celiac disease


  • Amna E. Al-araji Department of Pathological Analysis, University of Kafeel, Al-Najaf, Iraq.
  • Tuqa Z. Omran Department of Medical sciences, University of Al-Ameed, Kerbala, Iraq.
  • Mohanad M. Ahmed Department of medical microbiology and immunology, Kerbala University, Kerbala, Iraq.
  • Nazar J. Metib Consulting Pathologist, Al-Hussein Medical City, Kerbala, Iraq.




T-bet, Immunostaining, Gluten Sensitive Enteropathy, Distribution pattern, Marsh grading, Immunohistochemistry


Objective: In this study, we aimed to evaluate CD3, T-bet and GATA3 staining of intraepithelial lymphocytes (IELs) in comparison to the routine H&E stains in celiac disease.

Methods: a total of 50 patients, in whom celiac disease was diagnosed based on a combination of clinicopathological features, were enrolled in the study. Duodenal biopsied tissues were processed routinely into formalin fixed paraffin embedded (FFPE) blocks. Sections were prepared and stained with H&E and each of CD3, T-bet and GATA3. A number of histological criteria were measured to calculate the Marsh score. The results were analyzed using the IBM SPSS analytic software.

Results: a positive correlation was found between the count of T-bet stained cells and the increase of intraepithelial lymphocytes (P-value = 0.001). In addition, low count of GATA3 stained cells was seen in almost all cases. The count of GATA3 stained cells was not affected by the increase in IELs count.

Conclusions: the majority of increased IELs were stained with T-bet. Whereas in normal IELs count is less than half the IELs were stained with T-bet. This would indicate that T-bet immunostaining is a potential alternative to H&E and/or CD3 based counting of IELs.


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How to Cite

Al-araji, A. E., Omran, T. Z., Ahmed, M. M., & Metib, N. J. (2020). Evaluation of T-Bet immunostaining in the counting of intraepithelial lymphocytes in celiac disease. Journal of Contemporary Medical Sciences, 6(5), 229–233. https://doi.org/10.22317/jcms.v6i5.861