@article{Yaseen Mohammed Hasan_Alsamarrae_Rushdi_2023, title={Molecular Immuno-response Effects for Iraqi Lycium barbarm Carotenes upon Normal Human lymphocytes culture }, volume={9}, url={https://jocms.org/index.php/jcms/article/view/1335}, DOI={10.22317/jcms.v9i2.1335}, abstractNote={<p><strong>Objectives</strong>: The study aimed to investigate the effects of Iraqi <em>Lycium barbarm</em> Carotene on normal human blood lymphocytes at molecular level.</p> <p><strong>Methods:</strong> The normal human blood lymphocytes culture was exposed to Carotene extracted from Iraqi <em>Lycium barbarm</em> to estimate the interleukins  expression in treated lymphocytes represented by IL-10 and TNF-α as a moderator of immune response at a molecular scale, and their CD markers changes expression (CD3, CD4&CD8) by  flow-cytometer apparatus.</p> <p><strong>Results:</strong> Total carotenes content was 0.33mg/g powdered dried fruit. Potent carotene effect was appeared at concentration of 500µg/ml for  treated lymphocytes with increasing CD3 level to get upper level after 2 hours interval, while after 4 hours exposure both CD4 and CD8 levels increased dramatically for lymphocytes treated with 125 µg/ml, and 250µg/ml <em>Lycium </em>carotene. An alteration in cytokines gene expression for IL-10 production in response to carotene treatments at (125,250,500) µg/ml for both intervals, with suppress in tumor necrosis factor (TNF-α) gene expression in relation to β-actin the internal control and the PHA mitogenic agent.</p> <p><strong>Conclusion:</strong> The study concluded that the carotenes extracted from <em>Lycium barbarm</em> fruits seemed to initiate the immune system toward Th2 cell activation rather thanTh1, besides the extract may potentiate IL-10 production.  </p>}, number={2}, journal={Journal of Contemporary Medical Sciences}, author={Yaseen Mohammed Hasan, Zainab and Alsamarrae, Khulood W. and Rushdi, Ahmed}, year={2023}, month={Apr.} }