@article{Forouhandeh_Zarchini_Safarzadeh_Molavi_Asgharian_Tarhriz_2021, title={Evaluation of the Cytotoxic Activity of Anthriscus nemorosa on Breast Cancer Cells}, volume={7}, url={https://jocms.org/index.php/jcms/article/view/940}, DOI={10.22317/jcms.v7i2.940}, abstractNote={<p><strong>Objective:</strong> The present study concerns the cytotoxic activity of <em>A. nemorosa</em> different extracts on breast cancer cells  (MCF-7) and normal cell lines (HFFF).</p> <p><strong>Methods:</strong> Different extracts of aerial parts of  <em>A. nemorosa</em> were prepared using Soxhlet apparatus. The cytotoxicity of samples was assessed by MTT assay on breast cancer cells (MCF-7) and noncancerous cells (HFFF) with different concentrations of extracts in 24 and 48 hours. The most potent extract was fractioned and cytotoxic activity of fractions was considered, As well. A flow cytometry (annexin V/PI) assay has been used for detecting the mechanism of cell death in sample treated cell lines. Moreover, for clarifying volatile components of n-Hexane extract and its 80% and 100% VLC fractions were subjected to GC-MS apparatus.</p> <p><strong>Results</strong>: Results indicated that n-Hexane extract and its 80% and 100% VLC fractions exhibited a significant (p<0.001) inhibitory effect on the growth of the MCF-7 cell line compared to the control group. Meanwhile, flow cytometry analysis revealed that potent extract caused cell death through necrosis and 80% and 100% fractions showed different mechanisms (such as autophagy). The major compounds, which maybe were in charge of showing cytotoxic activity were non-terpenoids.</p> <p><strong>Conclusion: </strong>This study provides the evidence that<em> in vitro </em>cytotoxic activity of n-Hexane extract and 80% and 100% VLC fractions of <em>A. nemorosa </em>inhibited the proliferation of breast cancer cells (MCF7) via a different mechanism.</p>}, number={4}, journal={Journal of Contemporary Medical Sciences}, author={Forouhandeh, Haleh and Zarchini, Mahtab and Safarzadeh, Elham and Molavi, Omoleila and Asgharian, Parina and Tarhriz, Vahideh}, year={2021}, month={Aug.}, pages={217–226} }