TY - JOUR AU - Abdel-Hamid , Nabil Mohie AU - Hassan , Mohamed K AU - Ahmed, Amal AM AU - Abd Allah, Sara Gamal AU - Anber, Nahla H. PY - 2022/02/26 Y2 - 2024/03/29 TI - Liver proliferating cell nuclear antigen, BAX/Bcl-2 ratio, collagen, and polysaccharide accumulation as diagnostic tools in experimental hepatocellular carcinoma : BAX/Bcl-2 ratio, polysaccharide, collagen, and PCNA in hepatocellular carcinoma diagnosis JF - Journal of Contemporary Medical Sciences JA - J. Contemp. Med. Sci. VL - 8 IS - 1 SE - Articles DO - 10.22317/jcms.v8i1.1162 UR - https://jocms.org/index.php/jcms/article/view/1162 SP - 59-64 AB - <p><strong>Objectives:</strong>&nbsp; This experimental study was conducted to look for a sensitive diagnostic panel for early detection of HCC.</p><p><strong>Methods: </strong>Combination of diethyl nitrosamine (DENA, 200 mg/kg, IP, once), two weeks later, CCl4(3ml/kg/week), subcutaneously, for 6 weeks) induced HCC in rats. Sixteen male Wistar rats were divided into 2 groups, control, and HCC.</p><p><strong>Results:</strong> DENA plus CCl4 elevated serum alpha-fetoprotein, liver enzyme activity and depressed superoxide dismutase, reduced glutathione (GSH), elevated malondialdehyde. Also, depressed caspase-3, elevated collagen, polysaccharide accumulation, proliferating cell nuclear antigen in liver tissues, and depressed BAX / Bcl-2 ratio.&nbsp;&nbsp;&nbsp;</p><p><strong>Conclusion:</strong> Decreased BAX/Bcl-2 ratio, elevated collagen deposition, polysaccharide accumulation, nuclear proliferation, and tissue oxidative stress help in the early diagnosis of liver cancer.&nbsp;</p> ER -