Teratogenic effect of the aqueous extract of the Foeniculum vulgare (fennel) on fetal development in mice
DOI:
https://doi.org/10.22317/jcms.v5i5.657Keywords:
Foeniculum vulgare, Fennel, Teratogenicity, Embryotoxicity, Fetal Development, MiceAbstract
Objective The present study evaluated the effect of aqueous extract of Foeniculum vulgare on fetal development in pregnant mice models.
Methods A total number of 24 female BALB/c mice with a weight range of 25-30 gr divided into four groups. Each group received 0.25 ml of the aqueous extract of Foeniculum vulgare with different concentrations (2.5 mg, 12.5 mg, 25 mg) and distilled water as a control group. The aqueous extract of Foeniculum vulgare administered via oral gavage on a daily basis from day 6 to day 15 of pregnancy. On day 16, the fetuses were analyzed in terms of morphological changes, skeletal disorders, and cellular alterations.
Results The result showed the dose-dependent teratogenic effect of the aqueous extract of Foeniculum vulgare. At 12.5 mg and 25 mg concentration, the teratogenic effect was more severe. Oral gavage administration of the aqueous extract of Foeniculum vulgare increased the number of dead fetuses and reduced the average weight, height, and crown-rump length. Subcutaneous hemorrhage, dorsal lesion, wrinkled skin, and considerably lower than normal fetal weight observed in gross morphological inspection at 25 mg concentration. The skeletal studies revealed fetal anomalies, reduction in ossification and reduction in the number of ribs. Internal bleeding around the liver and lungs, pulmonary fibrosis and disruption in the arrangement of hepatocytes was also observed in histological analysis.
Conclusion Administration of the aqueous extract of Foeniculum vulgare results in embryotoxicity in mice models in morphological, skeletal and cellular levels.
Keywords Foeniculum vulgare, Fennel, Teratogenicity, Embryotoxicity, Fetal Development, Mice
References
2. Poorhassan M, Navae F, Mahakizadeh S, Bazrafkan M, Nikmehr B, Abolhassani F, et al. Flaxseed Can Reduce Hypoxia-Induced Damages in Rat Testes. International journal of fertility & sterility. 2018;12(3):235-41.
3. Sarmah S, Muralidharan P, Marrs JA. Common congenital anomalies: Environmental causes and prevention with folic acid containing multivitamins. Birth defects research Part C, Embryo today : reviews. 2016;108(3):274-86.
4. Minas A, Najafi G, Jalali AS, Razi M. Fennel induces cytotoxic effects against testicular germ cells in mice; evidences for suppressed pre-implantation embryo development. Environmental toxicology. 2018.
5. Khazaei M, Montaseri A, Khazaei MR, Khanahmadi M. Study of Foeniculum vulgare Effect on Folliculogenesis in Female Mice. Int J Fertil Steril. 2011;5(3):122-7.
6. Kooti W, Ali-Akbari S, Sharafi-Ahvazi N, Asadi-Samani M, ashtary-larky D. Therapeutic and pharmacological potential of Foeniculum vulgare Mill: A review. J HerbMed Pharmacol. 2015;4:1-9.
7. Badgujar SB, Patel VV, Bandivdekar AH. Foeniculum vulgare Mill: a review of its botany, phytochemistry, pharmacology, contemporary application, and toxicology. Biomed Res Int. 2014;2014:842674-.
8. Mesfin M, Asres K, Shibeshi W. Evaluation of anxiolytic activity of the essential oil of the aerial part of Foeniculum vulgare Miller in mice. BMC complementary and alternative medicine. 2014;14:310.
9. Kacaniova M, Mellen M, Vukovic NL, Kluz M, Puchalski C, Hascik P, et al. Combined Effect of Vacuum Packaging, Fennel and Savory Essential Oil Treatment on the Quality of Chicken Thighs. Microorganisms. 2019;7(5).
10. Juarez-Vazquez Mdel C, Carranza-Alvarez C, Alonso-Castro AJ, Gonzalez-Alcaraz VF, Bravo-Acevedo E, Chamarro-Tinajero FJ, et al. Ethnobotany of medicinal plants used in Xalpatlahuac, Guerrero, Mexico. Journal of ethnopharmacology. 2013;148(2):521-7.
11. Javidnia K, Dastgheib L, Mohammadi Samani S, Nasiri A. Antihirsutism activity of Fennel (fruits of Foeniculum vulgare) extract. A double-blind placebo controlled study. Phytomedicine : international journal of phytotherapy and phytopharmacology. 2003;10(6-7):455-8.
12. Joshi H, Parle M. Cholinergic basis of memory-strengthening effect of Foeniculum vulgare Linn. Journal of medicinal food. 2006;9(3):413-7.
13. Abou N, Abou El-Soud N, El-Laithy N, El-Saeed G, Wahby MS, Khalil M, et al. Antidiabetic Activities of Foeniculum Vulgare Mill. Essential Oil in Streptozotocin-Induced Diabetic Rats. Jun Macedonian Journal of Medical Sciences Jun Maced J Med Sci Jun. 2011;150173:139-146139.
14. Sadrefozalayi S, Farokhi F. Effect of the aqueous extract of Foeniculum vulgare (fennel) on the kidney in experimental PCOS female rats. Avicenna journal of phytomedicine. 2014;4(2):110-7.
15. Sadeghpour N, Khaki A, Najafpour A, Dolatkhah H, Montaseri A. Study of Foeniculum vulgare (Fennel) Seed Extract Effects on Serum Level of Estrogen, Progesterone and Prolactin in Mouse. Crescent Journal of Medical and Biological Sciences. 2015;2:23-7.
16. Hassanpour A, Yousefian S, Askaripour M, Sharififar F, Ezzatabadipour M. Ovarian protection in cyclophosphamide-treated mice by fennel. Toxicology reports. 2017;4:160-4.
17. Shokrzadeh M, Dashti A, Aghajanshakeri S, Pourabbas B, ghassemi barghi N. Prevention Effects of Foeniculum vulgare (Fennel) Hydroalcoholic Extract for Threatened Abortion by Misoprostol Induction in Experimental Mice. 2019;9:1-16.
18. Choi EM, Hwang JK. Antiinflammatory, analgesic and antioxidant activities of the fruit of Foeniculum vulgare. Fitoterapia. 2004;75(6):557-65.
19. Oktay M, Gülçin İ, Küfrevioğlu Öİ. Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. LWT - Food Science and Technology. 2003;36(2):263-71.
20. Abdel-Wahab A, Hashem Abdel-Razik AR, Abdel Aziz RL. Rescue effects of aqueous seed extracts of Foeniculum vulgare and Carum carvi against cadmium-induced hepatic, renal and gonadal damage in female albino rats. Asian Pacific journal of tropical medicine. 2017;10(12):1123-33.
21. Price KR, Fenwick GR. Naturally occurring oestrogens in foods--a review. Food additives and contaminants. 1985;2(2):73-106.
22. Zhang S, Chen X, Devshilt I, Yun Q, Huang C, An L, et al. Fennel main constituent, trans‑anethole treatment against LPS‑induced acute lung injury by regulation of Th17/Treg function. Mol Med Rep. 2018;18(2):1369-76.
23. Tognolini M, Ballabeni V, Bertoni S, Bruni R, Impicciatore M, Barocelli E. Protective effect of Foeniculum vulgare essential oil and anethole in an experimental model of thrombosis. Pharmacological research. 2007;56(3):254-60.
