Determination the Effectiveness of a selected formulations of Iranian Traditional Medicine “Ushaq poultice†(ammoniacum gum ointment) on Wound Healing: an experimental study
DOI:
https://doi.org/10.22317/jcms.v6i2.719Keywords:
wound healing, Dorema ammoniacum gum, herbal medicine, experimental studyAbstract
Objective: The purpose of this study was to evaluate the therapeutic effects of ammonium gum ointment on wound healing in the animal model.
Method: 32 male Wistar rats were selected; then in non-infectious condition, two full-thickness wounds with 8 mm in diameter were created bilaterally on shaved skin in 1.5 cm from the dorsal midline and randomly divided into four groups, untreated group, phenytoin cream treated group, base ointment (sesame oil and wax) treated group and Ammonicuma Gum Ointment (AGO) treated group. The animals received these medicines once daily. On days 5, 7, 10, 12 and 14 wound area was measured. On days 7 and 14, blood samples were taken, and serum level of growth factors (EGF, PDGF, VEGF, and TGF-B) were measured. Skin samples used for hematoxylin-eosin and trichrome staining. Skin tensile strength and hydroxyproline content of skin tissues also measured.
Results: The percentage of wound healing after 14 days of treatment was significantly increased in the group receiving AGO (PË‚0.05). Hydroxyproline content of repaired tissue and tensile strength increased considerably in this group (PË‚0.001). Growth factors were significantly increased in animals treated with AGO compared to control groups (EGF PË‚0.05; PDGF PË‚0.001; VEGF PË‚0.001; TGF-B PË‚0.05). Histological data showed that the topical application of AGO compared to other groups resulted in positive effects on enhance neovascularization and granulation, increased wound healing rate, and decreased wound size.
Conclusion: The topical application of an ointment containing ammoniacum gum can help speed up the process of wound healing.
References
1. Lazarus GS, Cooper DM, Knighton DR, Margolis DJ, Percoraro RE, Rodeheaver G, et al. Definitions and guidelines for assessment of wounds and evaluation of healing. Wound repair and regeneration. 1994;2(3):165-70.
2. Attinger CE, Janis JE, Steinberg J, Schwartz J, Al-Attar A, Couch K. Clinical approach to wounds: debridement and wound bed preparation including the use of dressings and wound-healing adjuvants. Plastic and reconstructive surgery. 2006;117(7S):72S-109S.
3. George Broughton I, Janis JE, Attinger CE. Wound healing: an overview. Plastic and reconstructive surgery. 2006;117(7S):1e-S-32e-S.
4. Hunt TK. The physiology of wound healing. Annals of emergency medicine. 1988;17(12):1265-73.
5. Robson MC, Steed DL, Franz MG. Wound healing: biologic features and approaches to maximize healing trajectories. Current problems in surgery. 2001;2(38):72-140.
6. Mirmalek SA, Parsa T, Parsa Y, Yadollah-Damavandi S, Salimi-Tabatabaee SA, Jangholi E, et al. The wound healing effect of Iris forentina on full thickness excisional skin wounds: A histomorphometrical study. Bangladesh Journal of Pharmacology. 2016;11(1):86-90.
7. Shen H-M, Chen C, Jiang J-Y, Zheng Y-L, Cai W-F, Wang B, et al. The N-butyl alcohol extract from Hibiscus rosa-sinensis L. flowers enhances healing potential on rat excisional wounds. Journal of ethnopharmacology. 2017;198:291-301.
8. Nicolaus C, Junghanns S, Hartmann A, Murillo R, Ganzera M, Merfort I. In vitro studies to evaluate the wound healing properties of Calendula officinalis extracts. Journal of ethnopharmacology. 2017;196:94-103.
9. Joshi A, Joshi VK, Pandey D, Hemalatha S. Systematic investigation of ethanolic extract from Leea macrophylla: Implications in wound healing. Journal of ethnopharmacology. 2016;191:95-106.
10. Pereira LdP, Mota MR, Brizeno LA, Nogueira FC, Ferreira EG, Pereira MG, et al. Modulator effect of a polysaccharide-rich extract from Caesalpinia ferrea stem barks in rat cutaneous wound healing: role of TNF-α, IL-1β, NO, TGF-β. Journal of ethnopharmacology. 2016;187:213-23.
11. Krishnappa P, Venkatarangaiah K, Rajanna SKS, Balan RK. Wound healing activity of Delonix elata stem bark extract and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside in rats. Journal of pharmaceutical analysis. 2016;6(6):389-95.
12. Öz BE, Ilhan M, Ozbilgin S, Akkol EK, Acikara ÖB, Saltan G, et al. Effects of Alchemilla mollis and Alchemilla persica on the wound healing process. Bangladesh Journal of Pharmacology. 2016;11(3):577-84.
13. MH AKS. qarabadin e kabir (great qarabadin). Tehran, Iran: Tehran University of medical science.
14. MH AKS. Makhzan-al-Advieh (The Storehouse Medicaments): Encyclopedia of Traditional Iranian Foods and Drugs (Old Times). . Calcutta, India: Tehran University of Medical Sciences; 1844. 1081 p.
15. Trease G, Evans W. Pharmacognosy (13th edn). Bailliere Tindall, London. 1989:176-80.
16. Mozaffarian V, Mozaffarian V, Mozaffarian V. Dictionary of Iranian plant names. 1996.
17. Langenheim JH. Plant resins: chemistry, evolution, ecology, and ethnobotany: Oregon, US: Timber Press; 2003.
18. Yousefzadi M, Heidari M, Akbarpour M, Mirjalili MH, Zeinali A, Parsa M. In vitro cytotoxic activity of the essential oil of Dorema ammoniacum D. Don. Middle-East J Sci Res. 2011;7(4):511-4.
19. Delnavazi M, Tavakoli S, Rustaie A, Batooli H, Yassa N. Antioxidant and antibacterial activities of the essential oils and extracts of Dorema ammoniacum roots and aerial parts. Research Journal of Pharmacognosy. 2014;1(4):11-8.
20. Bakhtiarian A, Shojaii A, Hashemi S, Nikoui V. Evaluation of analgesic and antiinflammatory activity of Dorema ammoniacum gum in animal model. Int J Pharm Sci Res. 2017;8(7):3102-6.
21. Kumar VP, Chauhan NS, Padh H, Rajani M. Search for antibacterial and antifungal agents from selected Indian medicinal plants. Journal of ethnopharmacology. 2006;107(2):182-8.
22. Motevalian M, Mehrzadi S, Ahadi S, Shojaii A. Anticonvulsant activity of Dorema ammoniacum gum: evidence for the involvement of benzodiazepines and opioid receptors. Research in pharmaceutical sciences. 2017;12(1):53.
23. Adhami H-R, Lutz J, Kählig H, Zehl M, Krenn L. Compounds from gum ammoniacum with acetylcholinesterase inhibitory activity. Scientia pharmaceutica. 2013;81(3):793-806.
24. SHAHIDI GH, Moein MR, Foroumadi AR, ROKHBAKHSH ZF. Cytotoxic activity of medicinal plants used in Iranian traditional medicine on two strains of Saccharomyces cerevisiae. 2002.
25. Cross S, Naylor L, Coleman R, Teo T. An experimental model to investigate the dynamics of wound contraction. British journal of plastic surgery. 1995;48(4):189-97.
26. Woessner Jr J. The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Archives of biochemistry and biophysics. 1961;93(2):440-7.
27. Edwards C, O'Brien Jr W. Modified assay for determination of hydroxyproline in a tissue hydrolyzate. Clinica chimica acta. 1980;104(2):161-7.
28. Fried NM, Walsh Jr JT. Laser skin welding: in vivo tensile strength and wound healing results. Lasers in Surgery and Medicine: The Official Journal of the American Society for Laser Medicine and Surgery. 2000;27(1):55-65.
29. Firdous SM, Sautya D. Medicinal plants with wound healing potential. Bangladesh Journal of Pharmacology. 2018;13(1):41-52.
30. Gosain A, DiPietro LA. Aging and wound healing. World journal of surgery. 2004;28(3):321-6.
31. George Broughton I, Janis JE, Attinger CE. The basic science of wound healing. Plastic and reconstructive surgery. 2006;117(7S):12S-34S.
32. Guo Sa, DiPietro LA. Factors affecting wound healing. Journal of dental research. 2010;89(3):219-29.
33. Young A, McNaught C-E. The physiology of wound healing. Surgery (Oxford). 2011;29(10):475-9.
34. Rajani M, Saxena N, Ravishankara M, Desai N, Padh H. Evaluation of the antimicrobial activity of ammoniacum gum from Dorema ammoniacum. Pharmaceutical biology. 2002;40(7):534-41.
35. Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomicâ€Canic M. Growth factors and cytokines in wound healing. Wound repair and regeneration. 2008;16(5):585-601.
36. De Masi ECDJ, Campos ACL, De Masi FDJ, Ratti MAS, Ike IS, De Masi RDJ. The influence of growth factors on skin wound healing in rats. Brazilian journal of otorhinolaryngology. 2016;82(5):512-21.
2. Attinger CE, Janis JE, Steinberg J, Schwartz J, Al-Attar A, Couch K. Clinical approach to wounds: debridement and wound bed preparation including the use of dressings and wound-healing adjuvants. Plastic and reconstructive surgery. 2006;117(7S):72S-109S.
3. George Broughton I, Janis JE, Attinger CE. Wound healing: an overview. Plastic and reconstructive surgery. 2006;117(7S):1e-S-32e-S.
4. Hunt TK. The physiology of wound healing. Annals of emergency medicine. 1988;17(12):1265-73.
5. Robson MC, Steed DL, Franz MG. Wound healing: biologic features and approaches to maximize healing trajectories. Current problems in surgery. 2001;2(38):72-140.
6. Mirmalek SA, Parsa T, Parsa Y, Yadollah-Damavandi S, Salimi-Tabatabaee SA, Jangholi E, et al. The wound healing effect of Iris forentina on full thickness excisional skin wounds: A histomorphometrical study. Bangladesh Journal of Pharmacology. 2016;11(1):86-90.
7. Shen H-M, Chen C, Jiang J-Y, Zheng Y-L, Cai W-F, Wang B, et al. The N-butyl alcohol extract from Hibiscus rosa-sinensis L. flowers enhances healing potential on rat excisional wounds. Journal of ethnopharmacology. 2017;198:291-301.
8. Nicolaus C, Junghanns S, Hartmann A, Murillo R, Ganzera M, Merfort I. In vitro studies to evaluate the wound healing properties of Calendula officinalis extracts. Journal of ethnopharmacology. 2017;196:94-103.
9. Joshi A, Joshi VK, Pandey D, Hemalatha S. Systematic investigation of ethanolic extract from Leea macrophylla: Implications in wound healing. Journal of ethnopharmacology. 2016;191:95-106.
10. Pereira LdP, Mota MR, Brizeno LA, Nogueira FC, Ferreira EG, Pereira MG, et al. Modulator effect of a polysaccharide-rich extract from Caesalpinia ferrea stem barks in rat cutaneous wound healing: role of TNF-α, IL-1β, NO, TGF-β. Journal of ethnopharmacology. 2016;187:213-23.
11. Krishnappa P, Venkatarangaiah K, Rajanna SKS, Balan RK. Wound healing activity of Delonix elata stem bark extract and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside in rats. Journal of pharmaceutical analysis. 2016;6(6):389-95.
12. Öz BE, Ilhan M, Ozbilgin S, Akkol EK, Acikara ÖB, Saltan G, et al. Effects of Alchemilla mollis and Alchemilla persica on the wound healing process. Bangladesh Journal of Pharmacology. 2016;11(3):577-84.
13. MH AKS. qarabadin e kabir (great qarabadin). Tehran, Iran: Tehran University of medical science.
14. MH AKS. Makhzan-al-Advieh (The Storehouse Medicaments): Encyclopedia of Traditional Iranian Foods and Drugs (Old Times). . Calcutta, India: Tehran University of Medical Sciences; 1844. 1081 p.
15. Trease G, Evans W. Pharmacognosy (13th edn). Bailliere Tindall, London. 1989:176-80.
16. Mozaffarian V, Mozaffarian V, Mozaffarian V. Dictionary of Iranian plant names. 1996.
17. Langenheim JH. Plant resins: chemistry, evolution, ecology, and ethnobotany: Oregon, US: Timber Press; 2003.
18. Yousefzadi M, Heidari M, Akbarpour M, Mirjalili MH, Zeinali A, Parsa M. In vitro cytotoxic activity of the essential oil of Dorema ammoniacum D. Don. Middle-East J Sci Res. 2011;7(4):511-4.
19. Delnavazi M, Tavakoli S, Rustaie A, Batooli H, Yassa N. Antioxidant and antibacterial activities of the essential oils and extracts of Dorema ammoniacum roots and aerial parts. Research Journal of Pharmacognosy. 2014;1(4):11-8.
20. Bakhtiarian A, Shojaii A, Hashemi S, Nikoui V. Evaluation of analgesic and antiinflammatory activity of Dorema ammoniacum gum in animal model. Int J Pharm Sci Res. 2017;8(7):3102-6.
21. Kumar VP, Chauhan NS, Padh H, Rajani M. Search for antibacterial and antifungal agents from selected Indian medicinal plants. Journal of ethnopharmacology. 2006;107(2):182-8.
22. Motevalian M, Mehrzadi S, Ahadi S, Shojaii A. Anticonvulsant activity of Dorema ammoniacum gum: evidence for the involvement of benzodiazepines and opioid receptors. Research in pharmaceutical sciences. 2017;12(1):53.
23. Adhami H-R, Lutz J, Kählig H, Zehl M, Krenn L. Compounds from gum ammoniacum with acetylcholinesterase inhibitory activity. Scientia pharmaceutica. 2013;81(3):793-806.
24. SHAHIDI GH, Moein MR, Foroumadi AR, ROKHBAKHSH ZF. Cytotoxic activity of medicinal plants used in Iranian traditional medicine on two strains of Saccharomyces cerevisiae. 2002.
25. Cross S, Naylor L, Coleman R, Teo T. An experimental model to investigate the dynamics of wound contraction. British journal of plastic surgery. 1995;48(4):189-97.
26. Woessner Jr J. The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Archives of biochemistry and biophysics. 1961;93(2):440-7.
27. Edwards C, O'Brien Jr W. Modified assay for determination of hydroxyproline in a tissue hydrolyzate. Clinica chimica acta. 1980;104(2):161-7.
28. Fried NM, Walsh Jr JT. Laser skin welding: in vivo tensile strength and wound healing results. Lasers in Surgery and Medicine: The Official Journal of the American Society for Laser Medicine and Surgery. 2000;27(1):55-65.
29. Firdous SM, Sautya D. Medicinal plants with wound healing potential. Bangladesh Journal of Pharmacology. 2018;13(1):41-52.
30. Gosain A, DiPietro LA. Aging and wound healing. World journal of surgery. 2004;28(3):321-6.
31. George Broughton I, Janis JE, Attinger CE. The basic science of wound healing. Plastic and reconstructive surgery. 2006;117(7S):12S-34S.
32. Guo Sa, DiPietro LA. Factors affecting wound healing. Journal of dental research. 2010;89(3):219-29.
33. Young A, McNaught C-E. The physiology of wound healing. Surgery (Oxford). 2011;29(10):475-9.
34. Rajani M, Saxena N, Ravishankara M, Desai N, Padh H. Evaluation of the antimicrobial activity of ammoniacum gum from Dorema ammoniacum. Pharmaceutical biology. 2002;40(7):534-41.
35. Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomicâ€Canic M. Growth factors and cytokines in wound healing. Wound repair and regeneration. 2008;16(5):585-601.
36. De Masi ECDJ, Campos ACL, De Masi FDJ, Ratti MAS, Ike IS, De Masi RDJ. The influence of growth factors on skin wound healing in rats. Brazilian journal of otorhinolaryngology. 2016;82(5):512-21.
Downloads
Published
2020-04-26
How to Cite
Zaheri Abdevand, Z., Siahpoosh, A., Malayeri, A., Salami, A., & Khorsandi, L. (2020). Determination the Effectiveness of a selected formulations of Iranian Traditional Medicine “Ushaq poultice†(ammoniacum gum ointment) on Wound Healing: an experimental study. Journal of Contemporary Medical Sciences, 6(2), 39–49. https://doi.org/10.22317/jcms.v6i2.719
Issue
Section
Articles
